ORIGIN AND FUNCTION OF TOMATO BUSHY STUNT VIRUS-INDUCED INCLUSION-BODIES .2. QUANTITATIVE ELECTRON-MICROSCOPE AUTORADIOGRAPHY AND IMMUNOGOLD CYTO-CHEMISTRY
ORIGIN AND FUNCTION OF TOMATO BUSHY STUNT VIRUS-INDUCED INCLUSION-BODIES .2. QUANTITATIVE ELECTRON-MICROSCOPE AUTORADIOGRAPHY AND IMMUNOGOLD CYTO-CHEMISTRY
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DOI：
10.1016/s0889-1605(86)80004-0
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发表时间：
1986-10-01
期刊：
影响因子：
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通讯作者：
DELLORTO, P
中科院分区：
文献类型：
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作者：
APPIANO, A;DAGOSTINO, G;DELLORTO, P
用氚化尿苷处理后的电镜放射自显影和免疫金标记技术研究了番茄丛矮病毒诱导的两种包涵体在Gomphrena globosa细胞中的可能作用。我们的观察表明，多泡体（MVB）和相关的叶绿体内陷都参与病毒复制。由于MBV总是存在于受感染的细胞中，即使在那些不含叶绿体的细胞中，它们似乎是病毒RNA合成的主要位点，而叶绿体内陷可能提供（当存在时）用于相同目的的额外囊泡位点。这两种结构都不含病毒蛋白。相反，致密颗粒不参与病毒RNA的合成，主要由病毒外壳蛋白组成，其产生可能发生在细胞质中。
The possible role of two types of inclusion bodies induced by tomato bushy stunt virus in Gomphrena globosa cells was investigated by electron microscope autoradiograpy after administration of tritiated uridine and by immunogold labeling by ultrathin sections. Our observations indicate that both multivesicular bodies (MVBs) and associated chloroplast invaginations are involved in virus replication. Since MBVs are always present in infected cells, even in those no containing chloroplasts, they appear to be the main site of virus RNA synthesis, while chloroplast invaginations are likely to provide, when present, an additional vesicular site for the same purpose. Neither of these structures contains viral protein. By contrast, the dense granules are not involved in virus RNA synthesis and consist mainly of virus coat protein, whose production is likely to occur in the cytoplasm.