An iron-deficient diet stimulates the onset of the hepatitis due to hepatic copper deposition in the Long-Evans Cinnamon (LEC) rat
An iron-deficient diet stimulates the onset of the hepatitis due to hepatic copper deposition in the Long-Evans Cinnamon (LEC) rat
复制标题
缺铁饮食会因长埃文斯肉桂 (LEC) 大鼠的肝脏铜沉积而刺激肝炎的发作
作者：
N. Sugawara;C. Sugawara
为研究饲料中铜、铁水平对Long-Evans Cinnamon（LEC）大鼠肝炎发病的影响，采用2 × 2析因设计，以含0.1或10 mg/kg铜和1.5或150 mg/kg铁的半纯化饲料喂养40日龄雌性LEC大鼠35天。在出生后75天，LEC大鼠（+Cu−Fe）喂养足够的铜，但缺乏铁的饮食（铜，10毫克/公斤;铁，1.5毫克/公斤）显示黄疸，嗜睡，厌食和不适。与其他三个组相比，与肝功能相关的生化变量显著增加，这三个组是Cu和Fe缺乏（−Cu−Fe）组、Cu缺乏但Fe充足（−Cu+Fe）组和Cu和Fe充足（+Cu+Fe）组。此外，+Cu-Fe大鼠肝脏显示出巨大的坏死细胞核。其他三组均未出现肝炎的生化和组织学结果。在+Cu-Fe大鼠中，肝脏Cu和金属硫蛋白浓度分别为289 ± 87（平均值±标准差）μg/g肝脏和8.7 ± 1.8 mg/g肝脏。而在+Cu+Fe组中，该值为196 ± 28 μg Cu/g肝和10.8 ± 1.0 mg/g肝。日粮铜水平对肝脏铁沉积无显著影响。黄疸+Cu−Fe组肝脏中游离Cu浓度最高，但肝脏游离Fe浓度与−Cu+Fe和+Cu+Fe组相似。我们的研究结果表明，由于胃肠道对铜的吸收增加，缺铁饮食会增加肝脏铜的沉积。这种沉积物刺激了肝炎的发作。
Abstract To study effects of dietary Cu and Fe levels on the onset of hepatitis in Long-Evans Cinnamon (LEC) rats, female rats (40 days old) were fed a semipurified diet containing 0.1 or 10 mg Cu/kg and 1.5 or 150 mg Fe/kg in a 2 × 2 factorial arrangement for 35 days. At 75 days after birth, LEC rats (+Cu−Fe) fed a Cu-sufficient but Fe-deficient diet (Cu, 10 mg/kg; Fe, 1.5 mg/kg) showed jaundice, with lethargy, anorexia, and malaise. The biochemical variables relating to liver function were significantly increased compared to three other groups, a Cu- and Fe-deficient (−Cu−Fe) group, a Cu-deficient but Fe-sufficient (−Cu+Fe) group, and a Cu and Fe sufficient (+Cu+Fe) group. Furthermore, the +Cu−Fe rat liver showed massive necrosis with huge nuclei. The other three groups presented no biochemical and histological findings of hepatitis. Hepatic Cu and metallothionein concentrations were 289 ± 87 (mean ± SD) μg/g liver and 8.7 ± 1.8 mg/g liver, respectively, in the +Cu−Fe rats. However, in the +Cu+Fe group the values were 196 ± 28 μg Cu/g liver and 10.8 ± 1.0 mg/g liver. Hepatic Fe deposition was not influenced significantly by the dietary Cu level. The +Cu−Fe group with jaundice showed the highest free Cu concentration in the liver among the four groups, but the hepatic free Fe concentration was similar to those in the −Cu+Fe and +Cu+Fe groups. Our results indicate that an Fe-deficient diet enhances the deposition of hepatic Cu due to increased absorption of Cu from the gastrointestinal tract. This deposition stimulated the onset of hepatitis.
我们对铁的吸收知之甚少。
DOI：
10.1093/ajcn/66.2.419
发表时间：
1997
期刊：
The American journal of clinical nutrition
影响因子：
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作者：
Beutler,E
通讯作者：
Beutler,E