DEVELOPMENT OF EMBRYONIC PATTERN IN DROSOPHILA-MELANOGASTER AS REVEALED BY ACCUMULATION OF THE NUCLEAR ENGRAILED PROTEIN
DEVELOPMENT OF EMBRYONIC PATTERN IN DROSOPHILA-MELANOGASTER AS REVEALED BY ACCUMULATION OF THE NUCLEAR ENGRAILED PROTEIN
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DOI：
10.1016/0092-8674(85)90012-1
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
OFARRELL, PH
中科院分区：
文献类型：
--
作者：
DINARDO, S;KUNER, JM;OFARRELL, PH
在果蝇的每个节段内建立和维持发育区室需要有网住。为了了解在这一过程中的作用，我们已经提出了针对enrailed的抗体，并通过间接免疫荧光观察胚胎中的enrailed蛋白。这种蛋白质在细胞核中积累，支持了“盘根错节”是一种调节因子的观点。第一种表达模式是在交替的片段中，然后在每个片段中表达，这表明enrailed可能对配对规则分割基因产物有反应。总体而言，在经历形态发生的区域中，成网蛋白水平达到峰值。最后，头部和末端的复杂的最终形式来自于早期的简单细分，这些领域的发展领域，通过盘根错节。
engrailed is required to establish and maintain developmental compartments within each segment of the fly. To understand the role of the engrailed protein in this process, we have raised antibodies against engrailed and have visualized an engrailed protein in embryos by indirect immunofluorescence. The protein accumulates in the nucleus, supporting the notion that engrailed is a regulatory factor. The first pattern of expression is in alternating segments followed by expression in every segment, suggesting that engrailed may be responding to pair-rule segmentation gene products. Overall, engrailed protein levels peak in areas undergoing morphogenesis. Finally, the complex final form of the head and terminalia derive from earlier simple subdivision of these areas into develomental fields by engrailed.