Bactericidal effect of tosufloxacin on Haemophilus influenzae internalized in cultured human cells.
Bactericidal effect of tosufloxacin on Haemophilus influenzae internalized in cultured human cells.
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托磺沙星对培养的人体细胞中内化的流感嗜血杆菌的杀菌作用。
DOI：
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Nuneki Hotomi
中科院分区：
文献类型：
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作者：
N. Yamanaka;Nuneki Hotomi
非分型流感嗜血杆菌是引起急性中耳炎(AOM)的主要病原菌之一，也被认为是导致儿童顽固性中耳炎(包括延长AOM和复发性AOM)的主要原因之一，其机制是将细菌内化到中耳粘膜上皮细胞。在这项研究中，我们可视化了流感嗜血杆菌在培养的人细胞中内化的动力学。我们还检测了抗菌药，包括新型喹诺酮类药物妥舒沙星和头孢类抗菌剂头孢妥仑对培养的人细胞内的流感嗜血杆菌的影响。结果表明：(1)流感嗜血杆菌内化进入人体细胞；(2)头孢妥仑对内化的流感嗜血杆菌无作用；(3)妥舒沙星对侵入人体细胞的流感嗜血杆菌有杀菌作用。这些数据有力地支持了托氟沙星治疗儿童顽固性中耳炎的高临床疗效。
Nontypeable Haemophilus influenzae, one of the major causative bacteria for acute otitis media (AOM), is also considered to cause intractable otitis media including prolonged AOM and recurrent AOM in children by the mechanism of internalization of the bacteria into epithelial cells of middle ear mucosa. In this study, we visualized the dynamics of H. infiuenzae internalization in cultured human cells. We also examined the effects of antimicrobials, including a novel quinolone, tosufloxacin, and a cephem antibacterial agent, cefditoren, on H. influenzae internalized in cultured human cells. The results indicated that (1) H. infiuenzae were internalized into human cells, (2) cefditoren has no effect on internalized H. influenzae, and (3) tosufloxacin has a bactericidal action on H. infiuenzae invading human cells. These data strongly support high clinical efficacy of tosuffoxacin on intractable otitis media in children.