Fndc5 overexpression facilitated neural differentiation of mouse embryonic stem cells
Fndc5 overexpression facilitated neural differentiation of mouse embryonic stem cells
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DOI：
10.1002/cbin.10427
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发表时间：
2015-05-01
影响因子：
3.9
通讯作者：
Nasr-Esfahani, Mohammad Hossein
中科院分区：
文献类型：
--
作者：
Forouzanfar, Mahboobeh;Rabiee, Farzaneh;Nasr-Esfahani, Mohammad Hossein
Fndc5是一种被切割并分泌到血液中的肌细胞因子。它被称为鸢尾素，在产热和能量稳态中起重要作用。据报道，在神经分化过程中，视黄酸处理后Fndc5的表达增加，其敲低降低了神经分化和神经突生长。本研究旨在探讨Fndc5过表达对小鼠神经分化率的影响。(Thus建立了在强力霉素作用下表达Fndc5的小鼠胚胎干细胞转基因细胞系。随后，研究了Fndc5的过表达对神经分化的不同阶段的影响）。我们的研究表明，Fndc5过表达后，神经元前体标记物和成熟神经元标记物增加增强，得出结论，Fndc5促进神经分化。这种效应可能与Fndc5过表达后BDNF表达增加有关。我们的研究结果与最近的研究报告Fndc5在神经细胞增殖和神经营养因子如BDNF表达增加中的类似作用一致。
Fndc5 has been recently recognized as a myokine which could be cleaved and secreted into blood stream. It is termed as irisin with an important role in thermogenesis and energy homeostasis. Increased expression of Fndc5 has been reported upon retinoic acid treatment during neural differentiation and its knockdown decreased neural differentiation and neurite outgrowth. This study tries to evaluate the effect of Fndc5 overexpression on rate of neural differentiation in mouse. (Thus, transduced cell line of mouse embryonic stem cell with ability to express Fndc5 under Doxycycline treatment was established. Subsequently, the effect of overexpression of Fndc5 on different stages of neural differentiation was studied). Our study showed an increase enhancement in neuronal precursor markers and mature neuron markers upon overexpression of Fndc5, concluding that Fndc5 facilitates neural differentiation. This effect might be related to increased expression of BDNF following overexpression of Fndc5. Our findings are consistent with recent studies reporting a similar role for Fndc5 in proliferation of neural cells and increase in the expression of neurotrophins like BDNF.