Trophic Effects of Aged Garlic Extract (AGE) and its Fractions on Primary Cultured Hippocampal Neurons from Fetal Rat Brain
Trophic Effects of Aged Garlic Extract (AGE) and its Fractions on Primary Cultured Hippocampal Neurons from Fetal Rat Brain
复制标题
陈蒜提取物 (AGE) 及其组分对胎鼠脑原代培养海马神经元的营养作用
DOI：
--
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
H. Katsuki
中科院分区：
文献类型：
--
作者：
T. Moriguchi;N. Nishiyama;H. Saito;H. Katsuki
研究了老化大蒜提取物及其组分对原代培养胎鼠大脑海马神经元存活及形态的影响。在培养基中添加AGE可显著提高培养海马神经元的存活率和每个轴突分支点的数量。AGE的神经营养活性存在于水溶性低分子量组分中，这是一种未精制的组分，含有94%的粗AGE成分。另一方面，从高分子量片段中纯化的蛋白质片段显示出有效的促进存活的作用，而对神经元形态没有任何影响。这些结果表明，AGE含有多种具有神经营养活性的成分，并且AGE及其组分是预防与年龄相关的中枢神经系统形态学变化的有用物质。
The effects of aged garlic extract (AGE) and its fractions were studied on the survival and morphology of the primary cultured hippocampal neurons from fetal rat brain. The addition of AGE to the culture medium significantly increased the survival of cultured hippocampal neurons and the number of branching points per axon. The neurotrophic activity of AGE resided in a water‐soluble‐low‐molecular‐weight fraction, which is an unrefined fraction that contains 94% of constituents in crude AGE. On the other hand, the protein fraction purified from a high‐molecular‐weight fraction, showed a potent survival‐promoting effect without any effect on the neuronal morphology. These results suggest that AGE contains multiple components which have neurotrophic activity and that AGE and its fractions are useful substances for preventing age‐related morphological changes in the central nervous system.