Artefacts in cell culture: α-Ketoglutarate can scavenge hydrogen peroxide generated by ascorbate and epigallocatechin gallate in cell culture media
Artefacts in cell culture: α-Ketoglutarate can scavenge hydrogen peroxide generated by ascorbate and epigallocatechin gallate in cell culture media
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DOI：
10.1016/j.bbrc.2011.01.091
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发表时间：
2011-03-04
影响因子：
3.1
通讯作者：
Halliwell, Barry
中科院分区：
文献类型：
--
作者：
Long, Lee Hua;Halliwell, Barry
抗坏血酸和几种酚类化合物在细胞培养基中很容易氧化产生过氧化氢。然而，添加 cc-酮戊二酸（已知多种细胞类型都会释放）会降低 H2O2 的水平，并且 α-酮戊二酸被耗尽并转化为琥珀酸。这些观察结果可以解释之前关于 α-酮戊二酸在促进培养细胞生长方面的保护作用的报道，并且可能有助于解释文献中报告的细胞培养系统中可自氧化化合物产生 H2O2 的速率的一些变异性。 (C) 2011 Elsevier Inc. 保留所有权利。
Ascorbate and several phenolic compounds readily oxidise in cell culture media to generate hydrogen peroxide. However, addition of cc-ketoglutarate, which is known to be released by several cell types, decreased the levels of H2O2, and the alpha-ketoglutarate was depleted and converted to succinate. These observations could account for previous reports of the protective effects of alpha-ketoglutarate in promoting the growth of cells in culture, and may contribute to explaining some of the variability in the literature in reported rates of H2O2 production from autoxidisable compounds in cell culture systems. (C) 2011 Elsevier Inc. All rights reserved.