Succinimidyl oleate, established inhibitor of CD36/FAT translocase inhibits complex III of mitochondrial respiratory chain
Succinimidyl oleate, established inhibitor of CD36/FAT translocase inhibits complex III of mitochondrial respiratory chain
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DOI：
10.1016/j.bbrc.2009.12.050
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发表时间：
2010-01-15
影响因子：
3.1
通讯作者：
Houstek, Josef
中科院分区：
文献类型：
--
作者：
Drahota, Zdenek;Vrbacky, Marek;Houstek, Josef
由于在CD36基因敲除小鼠中获得的结果与使用磺基N-琥珀酰亚胺油酸酯(SSO)抑制CD36介导的LCFA转运的实验相互矛盾，因此在线粒体组分中检测到的CD36蛋白在长链脂肪酸(LCFA)氧化中的功能作用尚不清楚：我们研究了SSO对线粒体呼吸的影响，发现SSO不仅显著抑制LCFA氧化，而且显著抑制依赖于黄蛋白和NADH的底物的氧化和线粒体膜电位的产生。在大鼠肝、心和肾线粒体的实验表明，对线粒体呼吸链有直接作用，其中对络合物III(IC50 4muM SSO)的抑制作用最明显。这些结果表明，SSO是一种有效的、不可逆转的线粒体呼吸链抑制物。(C)2010年，由爱思唯尔公司出版。
The functional role of CD36 protein detected in mitochondrial fractions in long chain fatty acid (LCFA) oxidation is unclear due to conflicting results obtained in Cd36 knockout mice and experiments using sulfo-N-Succinimidyl oleate (SSO) for inhibition of CD36 mediated LCFA transport: We investigated effect of SSO on mitochondrial respiration and found that SSO substantially inhibits not only LCFA oxidation, but also oxidation of flavoprotein- and NADH-dependent substrates and generation of mitochondrial membrane potential. Experiments in rat liver, heart and kidney mitochondria demonstrated a direct effect on mitochondrial respiratory chain with the most pronounced inhibition of the complex III (IC50 4 mu M SSO). The results presented here show that SSO is a potent and irreversible inhibitor of mitochondrial respiratory chain. (C) 2010 Published by Elsevier Inc.