Molecular Identification of the Kiss2/Kiss1ra System and Its Potential Function During 17Alpha-Methyltestosterone-Induced Sex Reversal in the Orange-Spotted Grouper, Epinephelus coioides
Molecular Identification of the Kiss2/Kiss1ra System and Its Potential Function During 17Alpha-Methyltestosterone-Induced Sex Reversal in the Orange-Spotted Grouper, Epinephelus coioides
复制标题
斜带石斑鱼 Kiss2/Kiss1ra 系统的分子鉴定及其在 17α-甲基睾酮诱导性逆转过程中的潜在功能
DOI：
10.1095/biolreprod.109.080044
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发表时间：
2010-07-01
影响因子：
3.6
通讯作者：
Lin, Haoran
中科院分区：
文献类型：
--
作者：
Shi, Yu;Zhang, Yong;Lin, Haoran
Kiss 1/Kiss 1 r系统是下丘脑-垂体-性腺（HPG）轴的一个组成部分，在调节促性腺激素和促性腺激素释放激素中起着重要作用。性反转过程是一种特殊的生殖现象，受HPG轴调控。为了更好地了解性逆转的神经内分泌机制，编码kiss 2和kiss 1 ra的cDNA已被克隆，并从橙点石斑鱼Epinealphus coioides，一种雌雄同体的硬骨鱼的功能特征。石斑鱼Kiss 2的核心成熟肽（Kiss 210）与其他KISS直系同源物具有高度相似性。在系统发育分析中，石斑鱼Kiss与硬骨鱼Kiss 2分支聚在一起，称为石斑鱼Kiss 2。石斑鱼kiss 1 ra的预测氨基酸序列在其N末端含有三个推定的糖基化位点，与其他硬骨鱼的氨基酸序列具有高度相似性。合成的Kiss 2 -10能够在培养的COS-7细胞中与Kiss 1 ra功能性地相互作用以触发下游信号传导。Kiss 2和Kiss 1 ra mRNA在所有组织中均表达，在嗅球中表达水平最高，在大脑区域中的下丘脑中表达水平最高，在外周组织中的卵巢中表达水平最高。腹腔注射Kiss 2 -10后6 h和12 h，下丘脑gnrh 1 mRNA水平和垂体fshb mRNA水平显著升高。在17 α-甲基睾酮（MT）诱导的石斑鱼性反转过程中，kiss 2和kiss 1 ra mRNA的表达在第1周显著降低，而kiss 2在第4周显著升高，与下丘脑gnrh 1 mRNA的表达模式一致。这是首次描述MT诱导的橘点石斑鱼性逆转过程中的Kiss 2/Kiss 1 ra系统。
The Kiss1/Kiss1r system is a component of the hypothalamus-pituitary-gonadal (HPG) axis, which plays a crucial role in regulating gonadotropins and gonadotropin-releasing hormone. The sex reversal process is a special reproductive phenomenon regulated by the HPG axis. To better understand the neuroendocrine mechanisms of sex reversal, cDNAs encoding kiss2 and kiss1ra have been cloned and functionally characterized from the orange-spotted grouper Epinephelus coioides, a protogynous hermaphroditic teleost. The core mature peptide (Kiss210) of grouper Kiss2 shared high similarity to other KISS orthologs. In phylogenetic analyses, the grouper Kiss was clustered with the teleost Kiss2 clade and termed grouper kiss2. The predicted amino acid sequence of grouper kiss1ra contained three putative glycosylation sites at its N-terminus, showing high similarity to that of other teleosts. Synthesized Kiss2-10 was able to functionally interact with Kiss1ra in cultured COS-7 cells to trigger downstream signaling. Both kiss2 and kiss1ra mRNAs were expressed in all tissues examined, with highest levels in the olfactory bulb and moderate levels in the hypothalamus among brain areas and highest levels in ovary among peripheral tissues. Intraperitoneal injection of Kiss2-10 significantly increased gnrh1 mRNA levels in hypothalamus and follicle-stimulating hormone beta (fshb) mRNA levels in the pituitary at 6 and 12 h postinjection. During the process of sex reversal induced by 17 alpha-methyltestosterone (MT), kiss2 and kiss1ra mRNA expression were significantly decreased in the first week, but kiss2 increased in the fourth week, in accordance with the expression pattern of gnrh1 mRNA in the grouper hypothalamus. This is the first description of the Kiss2/Kiss1ra system during MT-induced sex reversal in orange-spotted grouper.