On the Measurement of Gold Nanoparticle Sizes by the Dynamic Light Scattering Method
On the Measurement of Gold Nanoparticle Sizes by the Dynamic Light Scattering Method
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DOI：
10.1134/s1061933x11010078
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发表时间：
2011-02-01
期刊：
影响因子：
1.1
通讯作者：
Khlebtsov, N. G.
中科院分区：
文献类型：
--
作者：
Khlebtsov, B. N.;Khlebtsov, N. G.
讨论了动态光散射（DLS）法测定1-100 nm范围内胶体金纳米粒子粒径分布的应用。结果表明，当非球形强散射粒子的粒径大于30-40 nm时，其旋转扩散导致在5-10 nm范围内出现假峰。在这种情况下，DLS方法的非临界应用可能会产生与通过透射电子显微镜获得的颗粒体积或数量尺寸分布不同的颗粒。对于直径小于20 nm的弱散射颗粒，DLS方法证明了在大尺寸区域中的强度分布的附加峰，其与颗粒聚集体或副产物颗粒而不是单个纳米颗粒相关。讨论了解决假峰问题的实用方法。它是建立的DLS分布的宽度不对应于透射电子显微镜数据，被高估。的方法的优点和缺点进行了比较，并指出，目前，DLS方法是唯一的仪器适合于非扰动和敏感的诊断相对缓慢的聚集过程的特征时间为1分钟的顺序。特别是，这种方法可以用来诊断金纳米粒子共轭聚集引发的生物特异性相互作用在其表面上。
The application of the dynamic light scattering (DLS) method for determining the size distribution of colloidal gold nanoparticles in a range of 1-100 nm is discussed. It is shown that rotational diffusion of nonspherical strongly scattering particles with sizes of larger than 30-40 nm results in the appearance of a false peak in a size range of about 5-10 nm. In this case, the uncritical application of the DLS method may yield particle volume or number size distributions different from those obtained by transmission electron microscopy. For weakly scattering particles with diameters of smaller that 20 nm, the DLS method demonstrates an additional peak of intensity distribution in the region of large sizes that is related to particle aggregates or byproduct particles rather than individual nanoparticles. Practical methods for solving the problem of false peaks are discussed. It is established that the width of the DLS distribution does not correspond to transmission electron microscopy data and is overestimated. The advantages and drawbacks of the methods are compared and it is noted that, at present, the DLS method is the only instrument suitable for nonperturbative and sensitive diagnostics of relatively slow aggregation processes with characteristic times on the order of 1 min. In particular, this method can be used to diagnose gold nanoparticle conjugate aggregation initiated by biospecific interactions on their surface.