Muscle contractions induce interleukin-6 mRNA production in rat skeletal muscles
Muscle contractions induce interleukin-6 mRNA production in rat skeletal muscles
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DOI：
10.1111/j.1469-7793.2000.00157.x
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发表时间：
2000-10-01
影响因子：
5.5
通讯作者：
Pedersen, BK
中科院分区：
文献类型：
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作者：
Jonsdottir, IH;Schjerling, P;Pedersen, BK
1. 本研究探讨了白细胞介素 6 (IL-6) 可能因骨骼肌收缩而局部产生的假设，以及该产生是否可能反映肌肉收缩的类型。将大鼠麻醉，并用电刺激一侧肢体的小腿肌肉进行同心或偏心收缩（4 x 10 次收缩，4 个系列之间休息 1 分钟，100 Hz）。对侧肌肉作为未受刺激的对照。通过定量竞争性RT-PCR 对大鼠肌肉（白色和红色腓肠肌和比目鱼肌）中IL-6、倾斜：葡萄糖转运蛋白GLUT-4 和β-肌动蛋白的mRNA 水平进行定量。2。刺激后 30 分钟测量的 IL-G mRNA 水平在偏心和向心收缩后均有所增加，并且不同肌肉过滤类型之间的 IL-6 mRNA 水平没有显着差异。在未刺激的对侧肌肉fit，res.3中未观察到IL-6 mRNA水平的显着增加。没有检测到GLUT-4 mRNA水平的增加，表明IL-B mRNA水平的增加不是由于转录的一般变化所致。4．我们得出结论，IL-6是肌肉收缩后局部产生的，不同肌纤维类型之间没有显着差异。 IL-6 的局部产生并不是由于转录的一般变化，因为没有发现 GLUT-4 mRNA 水平的变化。在向心和离心收缩后都观察到 IL-6 mRNA 水平增加的事实：表明 IL-6 的产生不仅仅是由于肌肉损伤（主要在离心运动后出现）所致。
1. The present study explored the hypothesis that interleukin-6 (IL-6) might be locally produced in response to skeletal muscle contractions and whether the production might reflect the type of muscle contraction performed. Rats were anaesthetized and the calf muscles of one limb were stimulated electrically for concentric or eccentric contractions (4 x 10 contractions with 1 min of rest between the 4 series, 100 Hz). The contralateral muscles served as unstimulated controls. The mRNA levels for IL-6, tilt: glucose transport protein GLUT-4 and beta -actin in the rat muscles (white and red gastrocnemius and soleus) were quantified by quantitative competitive RT-PCR.2. The IL-G mRNA level, measured 30 min after the stimulation, increased after both eccentric and concentric contractions and there were no significant differences in IL-6 mRNA levels between the different muscle filtre types. No significant increase in IL-6 mRNA level was seen in the unstimulated contralateral muscle fit,res.3. No increase in GLUT-4 mRNA level was detected, indicating that the increase in IL-B mRNA level was not due to general changes in transcription.4. We conclude that IL-6 is locally produced after muscle contraction, with no significant differences between different muscle fibre types. This local production of IL-6 is not due to general changes in transcription, since no changes in the level of GLUT-4 mRNA were found. The fact that increased IL-6 mRNA levels were seen after both concentric and eccentric contractions: indicates that the production of IL-6 is not solely due to muscle damage, seen primarily after eccentric exercise.