Superparamagnetic Iron Oxide Nanoparticles as Magnetic Resonance Imaging Contrast Agents and Induced Autophagy Response in Endothelial Progenitor Cells
Superparamagnetic Iron Oxide Nanoparticles as Magnetic Resonance Imaging Contrast Agents and Induced Autophagy Response in Endothelial Progenitor Cells
复制标题
超顺磁性氧化铁纳米颗粒作为磁共振成像造影剂并诱导内皮祖细胞自噬反应
DOI：
10.1166/jbn.2019.2689
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发表时间：
2019-02-01
影响因子：
2.9
通讯作者：
Guo, Yingkun
中科院分区：
文献类型：
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作者：
Zhang, Lizhi;Jin, Rongrong;Guo, Yingkun
内皮祖细胞（Endothelial progenitor cells，EPCs）具有分泌血管生成因子和促进新生血管形成的功能，已被用于治疗心肌梗死、肢体缺血等缺血性疾病。然而，EPC治疗的临床应用依赖于建立一个无创，及时，有效，安全的评估系统。在这项研究中，我们招募了超顺磁性氧化铁纳米颗粒（SPION）增强磁共振成像（MRI）在体外跟踪EPCs。我们评估了SPION在EPCs中的标记效率，并确定了MRI标记细胞数量的阈值。我们的数据表明，用浓度为100 μ g Fe/mL的SPION孵育24小时的1x 10（5）EPCs可以用临床3 T MRI T-2扫描检测到。SPION对EPCs没有细胞毒性;然而，SPION诱导自噬和受损的新生血管形成。然后，我们研究了是否可以通过抑制自噬来减轻损伤。观察到的自噬反应及其在EPCs中的功能可能提供一种策略，以提高SPION标记的安全性。这也可能验证自噬的评估可能是一种更敏感的检测方法来评估追踪试剂的生物相容性。
Endothelial progenitor cells (EPCs) have been used in clinical trials for the treatment of patients with ischemic diseases, such as myocardial infarction and limb ischemia, as they could secrete angiogenic growth factors and promote neovascularization. However, the clinical employment of EPC therapy relies on the establishment of a noninvasive, timely, effective, and safe assessment system. In this study, we recruited superparamagnetic iron oxide nanoparticles (SPION) enhanced magnetic resonance imaging (MRI) to track EPCs in vitro. We evaluated the labeling efficiency of SPION in EPCs without cationic transfection reagents and determined the threshold value of the labeled cell quantity for MRI. Our data suggested that 1x10(5) EPCs incubated with SPION at a concentration of 100 mu g Fe/mL for 24 h could be detected with a clinical 3T MRI T-2 scan. SPION were not cytotoxic for EPCs; however, SPION induced autophagy and impaired neovascularization. We then examined if the impairment could be alleviated through the inhibition of autophagy. The observed autophagy response and its function in EPCs may provide a strategy to improve the safety of SPION labeling. It may also verify that the assessment of autophagy may be a more sensitive detection method to evaluate the biocompatibility of tracking reagents.