Blood meal source identification and RNA virome determination in Japanese encephalitis virus vectors collected in Ishikawa Prefecture, Japan, show distinct avian/mammalian host preference
Blood meal source identification and RNA virome determination in Japanese encephalitis virus vectors collected in Ishikawa Prefecture, Japan, show distinct avian/mammalian host preference
复制标题
DOI：
10.1093/jme/tjad028
复制
发表时间：
2023-04
影响因子：
2.1
通讯作者：
Astri Nur Faizah;Daisuke Kobayashi;Ryo Matsumura;M. Watanabe;Y. Higa;K. Sawabe;H. Isawa
中科院分区：
文献类型：
--
作者：
Astri Nur Faizah;Daisuke Kobayashi;Ryo Matsumura;M. Watanabe;Y. Higa;K. Sawabe;H. Isawa
在亚洲，库蚊特别令人感兴趣，因为它们在维持地方性蚊媒病毒性疾病（包括日本脑炎病毒（JEV））中的作用。尽管如此，宿主取食偏好，沿着自然感染RNA病毒在某些库蚊物种，仍然研究不足。在这项研究中，选定的吸血蚊子进行处理的鸟类和哺乳动物的血粉来源鉴定。同时，细胞培养繁殖和高通量测序（HTS）方法被用来确定在日本石川县收集的库蚊RNA病毒组。野外捕获库蚊血粉来源的鉴定。三带喙库蚊（Culex（Culex）tritaeniorhynchus Giles，1901）对野猪有强烈的偏好（62%，26/42），其次是苍鹭（21%，9/42）。另外两个物种，库蚊（Oculeomyia）bitaeniorhynchus Giles，1901年，和库蚊（库蚊）东方爱德华兹，1921年，表现出明显的偏好鸟类物种，包括候鸟。从HTS结果中，检测到34个病毒序列，其中4个是未分类的Aspiviridae、Qinviridae、Iflaviridae和Picornaviridae的新鉴定的病毒序列。在哺乳动物细胞中没有可观察到的细胞病变效应，系统发育分析表明，所有鉴定的病毒序列都是昆虫特异性的。进一步调查涉及其他蚊子种群收集在不同的地区是必要的，以探索以前未知的脊椎动物宿主，可能与JEV传播的性质。
In Asia, Culex mosquitoes are of particular interest because of their role in maintaining endemic mosquito-borne viral diseases, including the Japanese encephalitis virus (JEV). Nonetheless, host-feeding preferences, along with naturally infecting RNA viruses in certain Culex species, remain understudied. In this study, selected blood-fed mosquitoes were processed for avian and mammalian blood meal source identification. Concurrently, cell culture propagation and high-throughput sequencing (HTS) approaches were used to determine the RNA virome of Culex mosquitoes collected in Ishikawa Prefecture, Japan. The identification of blood meal sources from wild-caught Culex spp. revealed that Culex (Culex) tritaeniorhynchus Giles, 1901, has a robust preference toward wild boar (62%, 26/42), followed by heron (21%, 9/42). The other two species, Culex (Oculeomyia) bitaeniorhynchus Giles, 1901, and Culex (Culex) orientalis Edwards, 1921, showed a distinct preference for avian species, including migratory birds. From the HTS results, 34 virus sequences were detected, four of which were newly identified virus sequences of unclassified Aspiviridae, Qinviridae, Iflaviridae, and Picornaviridae. The absence of observable cytopathic effects in mammalian cells and phylogenetic analysis suggested that all identified virus sequences were insect-specific. Further investigations involving other mosquito populations collected in different areas are warranted to explore previously unknown vertebrate hosts that may be linked to JEV dispersal in nature.