Prp31p promotes the association of the U4/U6 x U5 tri-snRNP with prespliceosomes to form spliceosomes in Saccharomyces cerevisiae.
Prp31p promotes the association of the U4/U6 x U5 tri-snRNP with prespliceosomes to form spliceosomes in Saccharomyces cerevisiae.
复制标题
Prp31p 促进 U4/U6 x U5 tri-snRNP 与前剪接体的结合，在酿酒酵母中形成剪接体。
DOI：
10.1128/mcb.17.7.3580
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发表时间：
1997
影响因子：
5.3
通讯作者：
WoolfordJr,JL
中科院分区：
文献类型：
--
作者：
Weidenhammer,EM;Ruiz-Noriega,M;WoolfordJr,JL
ThePRP31gene encodes a factor essential for the splicing of pre-mRNA inSaccharomyces cerevisiae. Cell extracts derived from aprp31-1strain fail to form mature spliceosomes upon heat inactivation, although commitment complexes and prespliceosome complexes are detected under these conditions. Coimmunoprecipitation experiments indicate that Prp31p is associated both with the U4/U6 · U5 tri-snRNP and, independently, with the prespliceosome prior to assembly of the tri-snRNP into the splicing complex. Nondenaturing gel electrophoresis and glycerol gradient analyses demonstrate that while Prp31p may play a role in maintaining the assembly or stability of tri-snRNPs, functional protein is not essential for the formation of U4/U6 or U4/U6 · U5 snRNPs. These results suggest that Prp31p is involved in recruiting the U4/U6 · U5 tri-snRNP to prespliceosome complexes or in stabilizing these interactions.
