Flanking duplications and deletions associated with P-induced male recombination in Drosophila.
Flanking duplications and deletions associated with P-induced male recombination in Drosophila.
复制标题
果蝇中与 P 诱导的雄性重组相关的侧翼重复和缺失。
DOI：
10.1093/genetics/144.4.1623
复制
发表时间：
1996
期刊：
影响因子：
3.3
通讯作者：
Engels,WR
中科院分区：
文献类型：
--
作者：
Preston,CR;Sved,JA;Engels,WR
我们通过检查重组染色体的结构研究了Pelement诱导的生殖系有丝分裂细胞重组。我们发现，大多数重组体在重组位点保留了一个可移动的Pend元件，通常在发生交换的Pend附近有一个缺失或重复。这些缺失和重复的大小从几个碱基对到超过100 kb不等。这些结构符合雄性重组的“杂交元件插入”（HEI）模型，其中姐妹染色单体上的两个P-元件拷贝联合收割机结合形成“杂交元件”，其末端插入同源物上的邻近位置。这些数据表明，P诱导的重组可以作为一种有效的手段，产生侧翼缺失在现有的P元件附近。这些缺失很容易使用远侧侧翼标记筛选，并且可以根据选择哪种相互重组类型来选择它们以在给定方向上延伸。此外，可动P元件的保留允许通过随后的重组轮在该位点延伸缺失或产生额外的变异性。
We studiedPelement-induced recombination in germline mitotic cells by examining the structure of the recombinant chromosomes. We found that most recombinants retain a mobilePelement at the site of the recombination, usually with either a deletion or a duplication immediately adjacent to thePend at which the crossover occurred. The sizes of these deletions and duplications ranged from a few base pairs to well over 100 kb. These structures fit the “hybrid element insertion” (HEI) model of male recombination in which the twoP-element copies on sister chromatids combine to form a “hybrid element” whose termini insert into a nearby position on the homologue. The data suggest thatP-induced recombination can be used as an efficient means of generating flanking deletions in the vicinity of existingPelements. These deletions are easily screened using distant flanking markers, and they can be chosen to extend in a given direction depending on which reciprocal recombinant type is selected. Furthermore, the retention of a mobilePelement allows one to extend the deletion or generate additional variability at the site by subsequent rounds of recombination.