Improvement of the production of a red pigment in Penicillium sp HSD07B synthesized during co-culture with Candida tropicalis
Improvement of the production of a red pigment in Penicillium sp HSD07B synthesized during co-culture with Candida tropicalis
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与热带假丝酵母共培养期间合成的青霉菌 HSD07B 红色素产量的提高
DOI：
10.1016/j.biortech.2011.01.040
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发表时间：
2011-05-01
影响因子：
11.4
通讯作者：
Yao Jianming
中科院分区：
文献类型：
--
作者：
Wang Hailei;Ren Zhifang;Yao Jianming
青霉属（Penicillium sp.）HSD07B和热带假丝酵母（Candida tropicalis）的共培养产生了一种红色色素，通过薄层色谱（TLC）和高效液相色谱（HPLC）测定该色素由六种成分组成。该色素对小鼠无急性毒性，在Ames试验中无致突变性。该色素在pH 2 - 10以及10 - 100℃的温度范围内稳定，并且表现出良好的光稳定性以及对过氧化氢氧化和亚硫酸钠还原的抗性。接种物中的葡萄糖以及热带假丝酵母与HSD07B菌株的比例（w/w）是影响色素产生的重要因素。在优化条件下，摇瓶培养和151升生物反应器中分别获得了2.75克/升和7.7克/升的色素产量。因此，HSD07B菌株和热带假丝酵母的共培养是一种有前景的生产红色色素的方法，该色素可能在着色应用中有用。版权所有（C）2011，由爱思唯尔有限公司出版。保留所有权利。
Co-culture of Penicillium sp. HSD07B and Candida tropicalis resulted in the production of a red pigment consisting of six components as determined by TLC and HPLC. The pigment showed no acute toxicity in mice and was mot mutagenic in the Ames test. The pigment was stable between pH 2 and 10 and temperatures of 10-100 degrees C and exhibited good photo-stability and resistance to oxidization by hydrogen peroxide and reduction by Na(2)SO(3). Glucose and ratio of C. tropicalis to strain HSD07B (w/w) in the inoculum were the important factors influencing production of the pigment. Under optimized conditions, a pigment yield of 2.75 and 7.7 g/l was obtained in a shake-flask and a 151 bioreactor, respectively. Thus, co-culture of strain HSD07B and C. tropicalis is a promising way to produce a red pigment potentially useful for coloring applications. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.