Expression of Sox9 and type IIA procollagen during ocular development and aging in transgenic Del1 mice with a mutation in the type II collagen gene
Expression of Sox9 and type IIA procollagen during ocular development and aging in transgenic Del1 mice with a mutation in the type II collagen gene
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DOI：
10.1177/112067210201200602
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发表时间：
2002-11-01
影响因子：
1.7
通讯作者：
Salminen, H
中科院分区：
文献类型：
--
作者：
Ihanamäki, T;Säämänen, AM;Salminen, H
目的.研究转录因子Sox 9和IIA型前胶原在正常和转基因Dell小鼠眼发育和衰老过程中的表达和分布。在胚胎期E14.5、E16.5和E18.5，以及4和9个月大时，研究了戴尔小鼠的眼睛，并使用其非转基因同窝出生的小鼠作为对照。RNA酶保护试验和酶化学法检测Sox 9和前胶原（IIA）。RNA酶保护试验揭示了Sox9转录在戴尔和对照小鼠的眼睛在发育和老化。IIA型前胶原的mRNA具有相似的时间表达模式。在胚胎期E14.5、E16.5和E18.5，Sox 9通过免疫组织化学定位于细胞核中，IIA型前胶原定位于发育中的视网膜的细胞外间隙中。在生长和衰老过程中，眼Sox 9 mRNA的表达和Sox 9抗体的免疫组化反应减少，伴随着II型前胶原mRNA的减少。然而，在9个月龄时，Sox 9和IIA型前胶原mRNA的水平在戴尔和对照小鼠的退化眼中更高。Sox 9和IIA型前胶原蛋白的时空分布的相似性表明，这种转录因子参与了眼内II型胶原蛋白表达的激活，如在前软骨形成间充质和未成熟软骨中所证明的。在老化的视网膜和玻璃体中Sox 9和IIA型前胶原的增加的产生类似于退化的关节软骨，其中也已经观察到尝试的组织修复。
PURPOSE. To study the expression and distribution of transcription factor Sox9 and type IIA procollagen in the developing and aging eyes of normal and transgenic Dell mice carrying proalpha(II) collagen transgenes with a short deletion mutation, which cause ocular abnormalities in this mouse line.METHODS. The eyes of Dell mice were studied on embryonic days E14.5, E16.5 and E18.5, and at the ages of 4 and nine months, using their nontransgenic littermates as controls. Sox9 and proal(IIA) Collagen were detected by RNase protection assay and immunohistochemistry.RESULTS. RNase protection assay revealed Sox9 transcripts in the eyes of Dell and control mice during development and aging. The mRNA for type IIA procollagen had a similar temporal expression pattern. On embryonic days E14.5, E16.5 and E18.5, Sox9 was located by immunohistochemistry in the nuclei and type IIA procollagen in the extracellular space of the developing retina. During growth and aging, the ocular expression of Sox9 mRNA and the immunohistochemical reaction for Sox9 antibody diminished, concomitant with the reduction in type II procollagen mRNA. However, at the age of nine months, levels of Sox9 and type IIA procollagen mRNAs were higher in the degenerating eyes of Dell and control mice.CONCLUSIONS. The similarities in the temporo-spatial distribution of Sox9 and type IIA procollagen suggest that this transcription factor is involved in the activation of type II collagen expression in the eye, as has been demonstrated in prechondrogenic mesenchyme and immature cartilage. The increased production of Sox9 and type IIA procollagen in the aging retina and vitreous is analogous to degenerating articular cartilage where attempted tissue repair has also been observed.