A recombinant avian leukosis virus associated with fowl glioma in layer chickens in Japan
A recombinant avian leukosis virus associated with fowl glioma in layer chickens in Japan
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DOI：
10.1080/03079450801898815
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发表时间：
2008-01-01
期刊：
影响因子：
2.8
通讯作者：
Umemura, Takashi
中科院分区：
文献类型：
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作者：
Hatai, Hitoshi;Ochiai, Kenji;Umemura, Takashi
禽类胶质瘤是以星形胶质细胞多发结节状生长为特征的疾病，而禽类白血病病毒属的禽类胶质瘤诱导病毒是从日本板滩分离到的一种病原。自2003年以来，日本已报道了蛋鸡头部和颈部的皮下肿瘤，并在这些受影响的蛋鸡中同时发生了禽类胶质瘤。为明确鸡脑胶质瘤的蛋鸡特征，对240只鸡进行了组织病理学检查，其中皮下肿瘤18只，病鸡222只。光镜下，103层为非化脓性脑炎，4层为局限性广泛增生或星形胶质细胞多发结节。胶质瘤与皮下肿瘤共11层，独立发生在3层。此外，两层在大脑中有局部广泛的小而圆的细胞增殖。套式聚合酶链式反应未在病脑中检测到禽类胶质瘤诱导病毒基因组。从受影响的脑组织中分离到10株细菌。根据env基因的核苷酸序列分析，这些分离株的SU编码区与髓母细胞病相关病毒样病毒关系最密切，而TM区与内源性病毒(EV)基因座的相似性最高。其中一个分离株的基因组主要由EV基因组成，并含有其他禽白血病/肉瘤病毒的几个部分。这些结果表明，禽类胶质瘤的致病禽白血病病毒不仅是禽类胶质瘤诱导病毒，而且不同的禽白血病病毒株通过重组在中枢神经系统具有致瘤性，在日本是在蛋鸡中传播的。
Fowl glioma is characterized by multiple nodular growth of astrocytes, and fowl glioma-inducing virus belonging to avian leukosis virus has been isolated from Japanese bantam as a causal agent. Subcutaneous neoplasms of the head and neck have been reported in layer chickens since 2003 in Japan, and fowl glioma concurred in these affected layers. In the present study, the histopathology of 240 layers, including 18 layers with subcutaneous neoplasms and 222 layers kept with the affected layers, was performed to clarify the characteristics of fowl glioma in layers. Microscopically, 103 layers showed non-suppurative encephalitis, and four layers had locally extensive proliferation or multiple nodules of astrocytes. Gliomas concurred in 11 layers with subcutaneous neoplasms and occurred independently in three layers. In addition, two layers had locally extensive proliferation of small, round cells in the cerebrum. The fowl glioma-inducing virus genome was not detected in the affected brains by nested polymerase chain reaction. Ten isolates were obtained from the affected brains. By nucleotide sequencing of the env gene, SU coding regions of these isolates were most closely related to myeloblastosis-associated virus-like viruses, but TM regions showed the highest similarity to endogenous viral (ev) loci. The genome of one isolate mainly consisted of ev loci and contained several parts of other avian leukosis/sarcoma viruses. These results show that the causal avian leukosis virus of fowl glioma is not just fowl glioma-inducing virus and that different avian leukosis virus strains having oncogenicity in the central nervous system by recombination are spread in layers in Japan.