THE CARBOXYL-TERMINAL PART OF THE PUTATIVE BERNE VIRUS POLYMERASE IS EXPRESSED BY RIBOSOMAL FRAMESHIFTING AND CONTAINS SEQUENCE MOTIFS WHICH INDICATE THAT TORO-VIRUSES AND CORONAVIRUSES ARE EVOLUTIONARILY RELATED
THE CARBOXYL-TERMINAL PART OF THE PUTATIVE BERNE VIRUS POLYMERASE IS EXPRESSED BY RIBOSOMAL FRAMESHIFTING AND CONTAINS SEQUENCE MOTIFS WHICH INDICATE THAT TORO-VIRUSES AND CORONAVIRUSES ARE EVOLUTIONARILY RELATED
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DOI：
10.1093/nar/18.15.4535
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发表时间：
1990-08-11
影响因子：
14.9
通讯作者：
SPAAN, WJM
中科院分区：
文献类型：
--
作者：
SNIJDER, EJ;DENBOON, JA;SPAAN, WJM
对环状病毒原型伯尔尼病毒(Berne Virus，BEV)聚合酶基因3‘’部分(8kb)的序列分析表明，该区域至少含有两个开放阅读框(ORF1a和ORF1b)，它们重叠了12个核苷酸。测得ORF1b全序列(6873个核苷酸)。与冠状病毒一样，Bev在基因组RNA翻译过程中通过核糖体移码表达其ORF1b。所预测的Toro和冠状病毒移码区域的三级RNA结构(假结)相似。对预测的BeV ORF1b翻译产物的氨基酸序列分析表明，与冠状病毒的ORF1b产物具有同源性。确定了四个保守的结构域：假定的聚合酶结构域，包含保守的半胱氨酸和组氨酸残基的区域，假定的解旋酶基序，以及似乎是圆环和冠状病毒所独有的结构域。关于BeV聚合酶基因3‘’部分的数据补充了先前观察到的轮状病毒和冠状病毒在基因组组织和表达水平上的相似性。这两个病毒家族彼此之间的关系比与其他正链RNA病毒家族更密切。
Sequence analysis of the 3'' part (8 kb) of the polymerase gene of the torovirus prototype Berne virus (BEV) revealed that this area contains at least two open reading frames (provisionally designated ORF1a and ORF1b) which overlap by 12 nucleotides. The complete sequence of ORF1b (6873 nucleotides) was determined. Like the coronaviruses, BEV was shown to express its ORF1b by ribosomal frameshifting during translation of the genomic RNA. The predicted tertiary RNA structure (a pseudoknot) in the toro- and coronaviral frameshift-directing region is similar. Analysis of the amino acid sequence of the predicted BEV ORF1b translation product revealed homology with the ORF1b product of coronaviruses. Four conserved domains were identified: the putative polymerase domain, an area containing conserved cysteine and histidine residues, a putative helicase motif, and a domain which seems to be unique for toro- and coronaviruses. The data on the 3'' part of the polymerase gene of BEV supplement previously observed similarities between toro- and coronaviruses at the level of genome organization and expression. The two virus families are more closely related to each other than to other families of positive-stranded RNA viruses.