The association of transcribed genes with the nuclear matrix of Drosophila cells during heat shock.
The association of transcribed genes with the nuclear matrix of Drosophila cells during heat shock.
复制标题
热休克期间转录基因与果蝇细胞核基质的关联。
DOI：
10.1093/nar/13.7.2413
复制
发表时间：
1985
影响因子：
14.9
通讯作者：
Vogelstein,B
中科院分区：
文献类型：
--
作者：
Small,D;Nelkin,B;Vogelstein,B
利用热休克提供的转录调控，我们发现活性基因与核基质的关联并不依赖于它们的转录水平。热休克基因在热休克之前(当转录相对较低时)和在热休克期间(当转录大大增加时)都是基质相关的。相反，细胞质肌动蛋白基因在正常生长条件下(高转录时)和热休克期间(转录大大降低时)是基质相关的。在基质制备过程中去除99.7%以上的新生RNA并不影响这些发现。对细胞质肌动蛋白基因的详细研究表明，它的基质结合显然是由基因5‘端附近的多个相互作用介导的。
Using the transcriptionai modulation afforded by heat shock, we found that the association of active genes with the nuclear matrix was not dependent on their level of transcription. Heat shock genes were matrix associated both before heat shock (when transcription was relatively low), and during heat shock (when transcription was greatly increased). Conversely, the cytoplasmlc actin gene was matrix associated during normal growth conditions (when transcription was high) and during heat shock (when transcription was greatly decreased). Removal of greater than 99.7% of nascent RNA during preparation of the matrices did not affect these findings. Detailed examination of the cytoplasmlc actin gene revealed that its matrix association was apparently mediated by multiple interactions near the 5′ end of the gene.