THE J1-GLYCOPROTEIN - A NOVEL NERVOUS-SYSTEM CELL-ADHESION MOLECULE OF THE L2/HNK-1 FAMILY
THE J1-GLYCOPROTEIN - A NOVEL NERVOUS-SYSTEM CELL-ADHESION MOLECULE OF THE L2/HNK-1 FAMILY
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DOI：
10.1038/316146a0
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发表时间：
1985-01-01
期刊：
影响因子：
64.8
通讯作者：
SCHACHNER, M
中科院分区：
文献类型：
--
作者：
KRUSE, J;KEILHAUER, G;SCHACHNER, M
神经细胞黏附分子Li(参考文献1，2)和N-CAM(参考文献3-5)共享一个共同的碳水化合物表位，该表位被单抗L2和HNK-1(参考文献3-5)识别。6)。L2/HNK-1表位也存在于髓鞘相关糖蛋白(MAG)上，MAG被认为介导轴突和髓鞘细胞7之间的表面相互作用。其他尚未鉴定的细胞表面糖蛋白被这两种抗体识别，并被认为属于神经细胞黏附分子1，2，6家族。为了验证这一假设，我们制备了针对L2/HNK-1家族中重要成员160K(相对分子质量(MR)160,000)糖蛋白的多克隆抗体。在这里，我们报告这些抗体，命名为J1抗体，与星形胶质细胞和少突胶质细胞反应，干扰神经元与星形胶质细胞的黏附，但不干扰神经元与神经元或星形胶质细胞与星形胶质细胞的黏附。这一结果提示J1抗原参与了细胞间的相互作用。
The neural cell adhesion molecules LI (refs 1,2) and N-CAM (refs 3–5) share a common carbohydrate epitope that is recognized by the monoclonal antibodies L2 and HNK-1 (ref. 6). The L2/HNK-1 epitope is also present on the myelin-associated glycoprotein (MAG) which is thought to mediate surface interactions between the axon and myelinating cell7. Other, as yet unidentified, cell-surface glycoproteins are recognized by the two antibodies and are believed to belong to a family of neural cell adhesion molecules1,2,6. To test this hypothesis, we have prepared polyclonal antibodies to a prominent member of the L2/HNK-1 family, the 160K (relative molecular mass (Mr)160,000) glycoprotein. Here we report that these antibodies, designated J1 antibodies, react with astrocytes and oligodendrocytes and interfere with neurone–astrocyte adhesion, but not with neurone–neurone or astrocyte–astrocyte adhesion. This result suggests the involvement of the J1 antigen in cell–cell interactions.