Cells of different ploidy are often present together in Cryptococcus neoformans strains.
Cells of different ploidy are often present together in Cryptococcus neoformans strains.
复制标题
不同倍性的细胞经常一起存在于新型隐球菌菌株中。
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
K. Takeo
中科院分区：
文献类型：
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作者：
K. Hata;M. Ohkusu;S. Aoki;S. Ito‐Kuwa;K. Pienthaweechai;K. Takeo
我们对C.使用激光扫描细胞术和配备有光电倍增管的荧光显微镜两者来检测新生菌菌株。单倍体菌株由具有单倍体DNA量的正常大小的细胞组成。在细胞群中，也存在具有二倍体DNA量的大尺寸细胞。使用Skerman显微操作器分离单倍体菌株中的这些大细胞，进行培养，并且能够产生二倍体克隆。即使在斜面上仅转移3-5次后，在所有检查的二倍体克隆中也存在单倍体细胞。相反，通过从单倍体菌株中单细胞分离正常大小细胞获得的单倍体克隆在3-5次转移后也显示含有二倍体细胞。来自环境的新鲜单倍体分离物在3-5次转移后类似地含有二倍体细胞。因此，细胞倍性的转变广泛发生在C。neoformans菌株。
We examined the ploidy of C. neoformans strains using both laser scanning cytometry and a fluorescence microscope equipped with a photomultiplier. Haploid strains consisted of normal-sized cells with a haploid DNA amount. In the cell population there were also large-sized cells with a diploid DNA amount. These large cells in haploid strains were isolated using a Skerman micromanipulator, cultivated, and were able to generate diploid clones. Even after only 3-5 transfers on slants, haploid cells were present in all the diploid clones examined. Conversely, haploid clones obtained by single-cell-isolation of normal-sized cells from haploid strains were also shown to contain diploid cells after 3-5 transfers. Fresh haploid isolates from the environment similarly contained diploid cells after 3-5 transfers. Thus, a cellular ploidy shift was shown to occur widely in C. neoformans strains.