Development of a thin-film glucose biosensor using semiconducting amorphous carbons
Development of a thin-film glucose biosensor using semiconducting amorphous carbons
复制标题
使用半导体无定形碳开发薄膜葡萄糖生物传感器
DOI：
10.1016/0956-5663(95)96842-m
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发表时间：
1995
影响因子：
12.6
通讯作者：
L. J. Dunne
中科院分区：
文献类型：
--
作者：
A. D. Clark;M. Chaplin;S. Roulston;L. J. Dunne
磺胺酸在700 ~ 1400°K的温度范围内热分解形成的碳质材料在生物传感器中作为酶载体和电子介质具有相当大的潜力。这些碳具有多种用于酶结合的官能团，其电学和磁学性质已被广泛研究。本文介绍了一种葡萄糖薄膜生物传感器，该传感器是通过将葡萄糖氧化酶固定在1400°K制备的碳球上形成的。我们展示了该葡萄糖电极在1100 mV（相对于SCE）下在充气溶液中的电流响应；我们还展示了它的温度和pH依赖性，并报告了使用亚铁氰化物氧化还原系统的结果。
Carbonaceous materials formed by the thermal decomposition of sulphanilic acid in the temperature range 700 to 1400°K have considerable potential as enzyme supports and electron mediators in biosensors. These carbons have a variety of functional groups for enzyme binding and their electric and magnetic properties have been studied extensively. This paper describes a thin-film biosensor for glucose, formed by immobilizing glucose oxidase on pellets of the carbon prepared at 1400°K. We show the current response of this glucose electrode at 1100 mV (versus SCE) in aerated solutions; we also show its temperature and pH dependence, and report results using a ferrocyanide redox system.