Regulation of acetylcholine receptor synthesis at the level of translation in rat primary muscle cells.
Regulation of acetylcholine receptor synthesis at the level of translation in rat primary muscle cells.
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DOI：
10.1083/jcb.108.5.1817
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发表时间：
1989-05
影响因子：
7.8
通讯作者：
Salpeter, M M
中科院分区：
文献类型：
--
作者：
Horovitz, O;Spitsberg, V;Salpeter, M M
以前的研究表明，大鼠原代肌细胞不响应粗大鼠脑提取物或其活性成分之一，抗坏血酸，表面乙酰胆碱受体（AChR）的数量显着增加。我们在这里报告说，虽然很少或没有反应被认为是在细胞表面上，大鼠原代肌细胞响应粗脑提取物和抗坏血酸与AChR α亚基mRNA增加约三倍。mRNA的反应类似于在克隆的L5细胞中看到的。然而，虽然在L5细胞中α-亚基mRNA的增加进一步转化为α-亚基蛋白水平的增加，但在原代细胞中α-亚基合成没有这种增加。因此，这项研究表明，在α-亚基翻译水平的大鼠原代细胞表面乙酰胆碱受体合成的调节。这种调控水平不同于其他人报道的亚基转录或亚基组装。
Previous studies have shown that rat primary muscle cells do not respond to crude rat brain extract or one of its active components, ascorbic acid, with a significant increase in surface acetylcholine receptor (AChR) number. We report here that, although little or no response is seen on the cell surface, rat primary muscle cells do respond to both crude brain extract and to ascorbic acid with an approximately threefold increase in AChR alpha-subunit mRNA. The response of the mRNA is similar to that seen in the cloned L5 cells. However, while in L5 cells the increase in alpha-subunit mRNA is further translated into increased levels of alpha-subunit protein, there is no such increase in alpha-subunit synthesis in the primary cells. This study thus shows a regulation of surface AChR synthesis in rat primary cells at the level of alpha-subunit translation. This level of regulation is different from that involving subunit transcription or subunit assembly reported by others.