GLUTAMATE OXIDASE ENZYME ELECTRODES - MICROSENSORS FOR NEUROTRANSMITTER DETERMINATION USING ELECTROCHEMICALLY POLYMERIZED PERMSELECTIVE FILMS
GLUTAMATE OXIDASE ENZYME ELECTRODES - MICROSENSORS FOR NEUROTRANSMITTER DETERMINATION USING ELECTROCHEMICALLY POLYMERIZED PERMSELECTIVE FILMS
复制标题
DOI：
10.1016/0022-0728(95)03846-9
复制
发表时间：
1995-05-16
影响因子：
4.5
通讯作者：
PRITCHARD, DJ
中科院分区：
文献类型：
--
作者：
COOPER, JM;FOREMAN, PL;PRITCHARD, DJ
酶包埋在电化学聚合膜上是开发稳定、可重复、响应时间短的酶微传感器的一种有吸引力的方法。本文描述了一种用于测定谷氨酸的生物传感器的发展，该传感器涉及谷氨酸氧化酶在两种不同的电聚合基质中的包埋。随后，对不同基质中固定化酶的动力学进行了比较，并对聚合物在提供排除电位电活性干扰的超选择性屏障中的作用进行了表征。
Enzyme entrapment in electrochemically polymerized membranes is an attractive method for the development of stable reproducible enzyme microsensors with fast response times. In this paper the development of a biosensor for the determination of glutamate, involving the entrapment of the enzyme glutamate oxidase in two different electropolymerization matrices, is described. Subsequently, the kinetics of the immobilized enzyme in the different matrices are compared, and the role of the polymers in providing a permselective barrier for the exclusion of potential electroactive interferents are characterized.