Regeneration in the ears of immunodeficient mice: Identification and lineage analysis of mesenchymal stem cells
Regeneration in the ears of immunodeficient mice: Identification and lineage analysis of mesenchymal stem cells
复制标题
DOI：
10.1089/ten.2004.10.1251
复制
发表时间：
2004-07-01
影响因子：
--
通讯作者：
Gawronska-Kozak, B
中科院分区：
文献类型：
--
作者：
Gawronska-Kozak, B
裸鼠耳朵的伤口愈合类似于再生。对耳穿孔组织的组织学分析显示，在裸鼠中，最初形成的是胚芽样结构，随后是真皮、血管、软骨和肌肉的再生，而在野生型对照组(C57BL/6J)中没有。从再生(裸鼠)和非再生品系小鼠的外耳中分离出了一个干细胞亚群，称为耳间充质干细胞(EMSCs)。诱导胚胎间充质干细胞分化后的形态、组织化学和分子分析显示，所有品系的小鼠都有多种间充质细胞系(脂肪细胞、软骨细胞和骨细胞)，与它们的再生能力无关。因此，C57BL/6J野生型小鼠损伤耳再生的缺乏与组织培养中间充质干细胞分化的缺乏无关。由于裸鼠缺乏T淋巴细胞，在这个模型中，损伤耳朵中T淋巴细胞的缺失似乎提供了一个有利于间充质组织再生的微环境。这些发现为研究免疫系统对组织再生的影响提供了一个新的模型。
Wound healing in the ears of Athymic Nude-nu mice resembles regeneration. Histological analysis of the ear-punched tissues revealed the initial formation of a blastema-like structure followed by dermal, vascular, cartilage, and muscle regrowth exclusively in Athymic Nude-nu mice but not in wild-type controls (C57BL/6J). A subset of stem cells referred to here as ear mesenchymal stem cells (EMSCs) has been isolated from the external ears of regenerative (Athymic Nude-nu) and nonregenerative strains of mice. Morphological, histochemical, and molecular analysis after the induction of EMSC differentiation revealed multiple mesenchymal cell lineages (adipocytes, chondrocytes, and osteocytes) in all murine strains independent of their ability for regeneration. Thus, the absence of regeneration in wounded ears of C57BL/6J wild-type mice is not related to the absence of mesenchymal stem cell differentiation in tissue culture. Because nude mice lack T lymphocytes, it appears that in this model the absence of T lymphocytes in the wounded ears provides a microenvironment conducive to regeneration of mesenchymal tissues. These findings provide a new model to study the influence of the immune system on tissue regeneration.