Genetic analysis and CRISPR typing of Salmonella enterica serovar Enteritidis from different sources revealed potential transmission from poultry and pig to human
Genetic analysis and CRISPR typing of Salmonella enterica serovar Enteritidis from different sources revealed potential transmission from poultry and pig to human
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DOI：
10.1016/j.ijfoodmicro.2017.11.025
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发表时间：
2018-02-02
影响因子：
5.4
通讯作者：
Jiao, Xinan
中科院分区：
文献类型：
--
作者：
Li, Qiuchun;Wang, Xin;Jiao, Xinan
肠道沙门氏菌（S.沙门氏菌（沙门氏菌）是从家禽中分离的沙门氏菌中最流行的血清型之一，也是人类沙门氏菌病最常见的报道原因。本研究旨在对329株S. 2009年至2016年中国不同来源分离的肠杆菌菌株。使用规则间隔短回文重复序列（CRISPR）分型来表征这262个鸡临床分离株、38个人分离株、18个猪分离株、6个鸭分离株、3个鹅分离株和2个未知来源的分离株。共鉴定出18种肠道CRISPR类型（ECTs），其中ECT 2、ECT 8和ECT 4为前三种ECTs。CRISPR分型确定ECT 2是最常见的ECT，占S.除鸭外，所有来源的肠道菌株。在猪和人分离株中鉴定出了ECT 9和ECT 13，并揭示了从猪到人的潜在传播。聚类分析将包括前三名在内的18个ECT分为四个谱系，LI为主要谱系。329个分离株中有48个进行了全基因组序列分型，将它们分为四个簇，其中簇I为主要簇。聚类I包括从CRISPR分型鉴定的位于LI中的92%（34/37）的菌株，证实了两种分型方法之间的良好对应性。此外，CRISPR分型还揭示了ECTs与隔离区域之间的密切关系，证实了CRISPR间隔区可能是细菌从环境中独特的噬菌体或质粒库中获得的。然而，还需要进一步的分析来确定CRISPR-Cas系统在沙门氏菌中的功能以及间隔区与环境之间的关系。
Salmonella enterica serovar Enteritidis (S. Enteritidis) is one of the most prevalent serotypes in Salmonella isolated from poultry and the most commonly reported cause of human salmonellosis. In this study, we aimed to assess the genetic diversity of 329 S. Enteritidis strains isolated from different sources from 2009 to 2016 in China. Clustered regularly interspaced short palindromic repeat (CRISPR) typing was used to characterize these 262 chicken clinical isolates, 38 human isolates, 18 pig isolates, six duck isolates, three goose isolates and two isolates of unknown source. A total of 18 Enteritidis CRISPR types (ECTs) were identified, with ECT2, ECT8 and ECT4 as the top three ECTs. CRISPR typing identified ECT2 as the most prevalent ECT, which accounted for 41% of S. Enteritidis strains from all the sources except duck. ECT9 and ECT13 were identified in both pig and human isolates and revealed potential transmission from pig to human. A cluster analysis distributed 18 ECTs, including the top three ECTs, into four lineages with LI as the predominant lineage. Forty-eight out of 329 isolates were subjected to whole genome sequence typing, which divided them into four clusters, with Cluster I as the predominant cluster. Cluster I included 92% (34/37) of strains located in LI identified from the CRISPR typing, confirming the good correspondence between both typing methods. In addition, the CRISPR typing also revealed the close relationship between ECTs and isolated areas, confirming that CRISPR spacers might be obtained by bacteria from the unique phage or plasmid pools in the environment. However, further analysis is needed to determine the function of CRISPR-Cas systems in Salmonella and the relationship between spacers and the environment.