A strong nucleotypic effect on the cell cycle regardless of ploidy level
A strong nucleotypic effect on the cell cycle regardless of ploidy level
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DOI：
10.1093/aob/mcn038
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发表时间：
2008-04-01
期刊：
影响因子：
4.2
通讯作者：
Barlow, Peter W.
中科院分区：
文献类型：
--
作者：
Francis, Dennis;Davies, M. Stuart;Barlow, Peter W.
背景和目的在已发表的研究中，被子植物的核型和有丝分裂细胞周期的持续时间之间的正相关关系已被报道，但分析的物种数量最多的是大约。60.在这里提出的DNA C-值和细胞周期时间的被子植物包括110个测量，包括单子叶植物和真双子叶植物在设定的温度范围内，并包括一个大约。290-方法将110个已发表的在20-25摄氏度之间的温度下生长的幼苗的细胞周期时间的数据与DNA C值（单子叶植物58个值，真双子叶植物52个值）进行比较。对所有物种进行了回归分析，并分别对单子叶植物和真双子叶植物，二倍体和多倍体，以及一年生植物和多年生植物进行了回归分析。细胞周期时间对细胞核DNA C值作图。关键结果DNA C值和细胞周期时间之间的正相关关系，观察到所有物种和真双子叶植物和单子叶植物分别，无论存在或不存在的多倍体值。在该样品中，52个真双子叶植物中的最大细胞周期长度为18 h，而58个单子叶植物的最大细胞周期长度为8-120 h。有一个显着的额外增加，在多年生单子叶植物的细胞周期的持续时间与C值大于25 pg.的确，最强大的DNA C值和细胞周期时间和细胞周期时间的范围最广的关系是在多年生无论倍性level.Conclusions DNA复制被确定为细胞周期中的限速步骤，DNA复制的灵活性进行了探讨，我们推测，DNA复制起始点的许可可能是C值对有丝分裂细胞周期持续时间的正核型效应的响应成分。
Bckground and Aims In published studies, positive relationships between nucleotype and the duration of the mitotic cell cycle in angiosperms have been reported but the highest number of species analyzed was approx. 60. Here an analysis is presented of DNA C-values and cell cycle times in root apical meristems of angiosperms comprising 110 measurements, including monocots and eudicots within a set temperature range, and encompassing an approx. 290-fold variation in DNA C-values.Methods Data for 110 published cell cycle times of seedlings grown at temperatures between 20-25 degrees C were compared with DNA C-values (58 values for monocots and 52 for eudicots). Regression analyses were undertaken for all species, and separately for monocots and eudicots, diploids and polyploids, and annuals and perennials. Cell cycle times were plotted against the nuclear DNA C-values.Key Results A positive relationship was observed between DNA C-value and cell cycle time for all species and for eudicots and monocots separately, regardless of the presence or absence of polyploid values. In this sample, among 52 eudicots the maximum cell cycle length was 18 h, whereas the 58 monocot values ranged from 8-120 h. There was a striking additional increase in cell cycle duration in perennial monocots with C-values greater than 25 pg. Indeed, the most powerful relationship between DNA C-value and cell cycle time and the widest range of cell cycle times was in perennials regardless of ploidy level.Conclusions DNA replication is identified as a rate limiting step in the cell cycle, the flexibility of DNA replication is explored, and we speculate on how the licensing of initiation points of DNA replication may be a responsive component of the positive nucleotypic effect of C-value on the duration of the mitotic cell cycle.