Role of substance P (SP) in development of symptoms of neurogenic inflammation in the oral mucosa of the rat.
Role of substance P (SP) in development of symptoms of neurogenic inflammation in the oral mucosa of the rat.
复制标题
P 物质 (SP) 在大鼠口腔粘膜神经源性炎症症状发展中的作用。
DOI：
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发表时间：
1993
影响因子：
3.5
通讯作者：
L. Rosivall
中科院分区：
文献类型：
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作者：
A. Györfi;Á. Fazekas;F. Irmes;G. Jakab;T. Sütö;L. Rosivall
在本系列研究中，我们首先研究了外源性 P 物质 (SP) 对大鼠口腔粘膜神经源性炎症特征（即血管通透性和血流量）的局部影响。研究表明，用辣椒素进行预处理可以减轻神经源性炎症的症状。因此，我们还使用免疫组织化学技术，在对照大鼠以及新生儿和成年期用辣椒素预处理的动物中评估了下颌粘膜中显示 P 物质样免疫反应性 (SP-IR) 的神经纤维的分布。与对照值相比，以 7.5 nmol 剂量施用 SP 导致血管通透性 (NS) 增加近 70%，而施用四倍高剂量（30 nmol）则导致伊文思蓝外渗增加约 150%（p < 0.05）。观察到 45 nmol SP 溶液对血管通透性的类似增加（约 146%）（p < 0.05）。虽然 7.5 nmol SP 溶液未能影响血流，但 30 nmol SP 显着增加了血流约 10%。 38%（p < 0.05）。施用 45 nmol SP 溶液导致血流类似的增强（43%，p < 0.05）。在新生儿或成年期进行的辣椒素预处理减少了口腔粘膜中 SP 免疫反应性纤维的数量。我们的功能结果表明，SP 可能在实验诱导的大鼠口腔粘膜神经源性炎症中发挥作用。我们的发现也支持了这一点，即辣椒素预处理可以减少这些组织中 SP 免疫反应性纤维的数量，从而减轻神经源性炎症的症状。
In the present series of investigations we first studied the local effects of exogenous substance P (SP) on the hallmarks of neurogenic inflammation, i.e. vascular permeability and blood flow, in the oral mucosa of the rat. Pretreatment with capsaicin was shown to attenuate the symptoms of neurogenic inflammation; therefore, the distribution of nerve fibers displaying substance P-like immunoreactivity (SP-IR) in the mandibular mucosa was also assessed in control rats and in animals pretreated with capsaicin both neonatally and in adulthood using immunohistochemical techniques. The application of SP at a dose of 7.5 nmol resulted in an almost 70% increase of vascular permeability (NS) and the administration of a four-fold higher dose (30 nmol) produced about 150% increase in Evans blue extravasation compared with control values (p < 0.05). A similar increase (ca 146%) in vascular permeability was observed in response to 45 nmol SP solution (p < 0.05). While the 7.5 nmol SP-solution failed to affect blood flow, the 30 nmol SP significantly increased it by ca. 38% (p < 0.05). The administration of the 45 nmol SP solution resulted in a similar enhancement of blood flow (43%, p < 0.05). Capsaicin pretreatment performed either neonatally or in adulthood has reduced the number of SP-immunoreactive fibers in the oral mucosa. Our functional results suggest that SP may have a role in the experimentally-induced neurogenic inflammation of the oral mucosa in the rat. This is also supported by our finding that capsaicin pretreatment, known to decrease the number of SP-immunoreactive fibers in these tissues, reduced the symptoms of neurogenic inflammation.