In Vitro and In Vivo Biocompatibility Analysis of a New Transparent Collagen-based Wound Membrane for Tissue Regeneration in Different Clinical Indications
In Vitro and In Vivo Biocompatibility Analysis of a New Transparent Collagen-based Wound Membrane for Tissue Regeneration in Different Clinical Indications
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DOI：
10.21873/invivo.12040
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发表时间：
2020-09-01
期刊：
影响因子：
2.3
通讯作者：
Barbeck, Mike
中科院分区：
文献类型：
--
作者：
Jung, Ole;Radenkovic, Milena;Barbeck, Mike
背景/目的：对于不同组织缺损的治疗，例如颌骨缺损、开放性伤口缺损、慢性溃疡、硬脑膜缺损和角膜缺损，可使用不同的生物材料。在过去的几十年中，由于其优异的生物相容性和可降解性，胶原基材料在这些应用中的使用显著增加。然而，直到现在还没有透明的基于胶原蛋白的生物材料。因此，一种新开发的透明胶原蛋白膜（TCM）的基础上，天然衍生的猪心包膜，它提供了许多应用的可能性，开发。本研究旨在使用已建立的方法分析体外和体内生物相容性。材料与方法：对新型TCM膜和市售胶原蛋白膜（CM，Jason膜，botiss biomaterials GmbH，Zossen，德国）进行体外细胞相容性试验。以假手术为对照组，分析其体内生物相容性。在体外，根据EN ISO 10993-5/-12法规和活-死-染色测试细胞相容性。在体内，使用BALB/c小鼠皮下植入模型，并通过建立的组织学、免疫组织化学和组织形态计量学方法制备外植体用于分析。结果如下：在体外，这两种膜显示出有希望的细胞相容性，在TCM的活-死染色试验中具有稍好的直接细胞反应。在体内，TCM在10天和30天后诱导了相当的炎症免疫应答，其中MI-和M2-巨噬细胞的数量相当，这也在没有生物材料插入的对照组中发现。结论：新型透明胶原膜具有良好的生物相容性，可安全应用于组织修复和外科手术。
Background/Aim: For the treatment of different tissue defects such as jawbone defects, open wound defect, chronic ulcers, dura mater defects and corneal defects, different biomaterials are available. The use of collagen-based materials for these applications has been significantly increased over the past decades due to its excellent biocompatibility and degradability. However, no transparent collagen-based biomaterial is available until now. Thus, a newly developed transparent collagen membrane (TCM) based on natural derived porcine pericardium, which offers numerous application possibilities, was developed. The present study aimed to analyze the in vitro and in vivo biocompatibility using established methods. Materials and Methods: The new TCM membrane and a commercially available collagen membrane (CM, Jason membrane, botiss biomaterials GmbH, Zossen, Germany) were tested for its in vitro cytocompatibility. Furthermore, the in vivo biocompatibility was analyzed using sham operations as control group. In vitro, cytocompatibility was tested in accordance with EN ISO 10993-5/-12 regulations and Live-Dead-stainings. In vivo, a subcutaneous implantation model in BALB/c mice was used and explants were prepared for analyses by established histological, inununohistochemical and histomorphometrical methods. Results: In vitro, both membranes showed promising cytocompatibility with a slightly better direct cell response in the Live-Dead staining assay for the TCM. In vivo, TCM induced a comparable inflammatory immune response after 10 and 30 days with comparable numbers of MI- and M2-macrophages as also found in the control group without biomaterial insertion. Conclusion: The newly transparent collagen membrane is filly biocompatible and is supporting safe clinical application in tissue repair and surgery.