Serum levels of nicotinamide N-methyltransferase in patients with lung cancer
Serum levels of nicotinamide N-methyltransferase in patients with lung cancer
复制标题
DOI：
10.1007/s00432-009-0563-y
复制
发表时间：
2009-09-01
影响因子：
3.6
通讯作者：
Akiyama, Hirohiko
中科院分区：
文献类型：
--
作者：
Tomida, Mikio;Mikami, Iwao;Akiyama, Hirohiko
本研究的目的是确定烟酰胺n -甲基转移酶（NNMT）是否可作为肺癌（I-III期）的生物标志物。建立了NNMT酶联免疫吸附试验系统。我们测定了113例接受手术的非小细胞肺癌（NSCLC）患者、50例合并慢性阻塞性肺疾病（COPD）的非肿瘤性肺病患者和24名健康供者血清中NNMT和癌胚抗原（CEA）的水平。肺癌患者血清NNMT水平明显高于COPD患者和健康供者。通过受体-工作特征曲线分析NNMT和CEA检测肺癌的特异性和敏感性之间的关系。NNMT的曲线下对应面积为0.703，CEA的曲线下对应面积为0.621，说明NNMT的灵敏度稍好。NNMT和CEA作为肺癌标志物的敏感性分别为25%和24%，特异性为90%。NNMT与CEA无显著相关。因此，当NNMT与CEA联合使用时，90%特异性的NSCLC检测灵敏度从25%提高到32%。血清NNMT水平对NSCLC患者的早期发现可能具有重要意义。
The aim of this study was to determine if nicotinamide N-methyltransferase (NNMT) is useful as a biomarker of lung cancer (stages I-III).We established an ELISA system for NNMT. We determined the levels of NNMT and carcinoembryonic antigen (CEA) in sera of 113 non-small cell lung cancer (NSCLC) patients undergoing surgery and sera of 50 non-neoplastic lung disease patients with chronic obstructive pulmonary disease (COPD) and of 24 healthy donors.The serum levels of NNMT were significantly higher in lung cancer patients than in COPD patients and healthy donors. The relationship between the specificity and sensitivity of NNMT and CEA measurements for the detection of lung cancer was analyzed by means of receiver-operating characteristic curves. The corresponding areas under the curves were 0.703 for NNMT and 0.621 for CEA, indicating slightly better sensitivity of NNMT. With 90% specificity, the sensitivities of NNMT and CEA as lung cancer markers were 25 and 24%, respectively. There was no significant correlation between NNMT and CEA. Therefore, the sensitivity of NSCLC detection at 90% specificity increased from 25 to 32% when NNMT was used in combination with CEA.The NNMT serum level may have significance in the early detection of NSCLC patients.