In vitro cell alignment obtained with a Schwann cell enriched microstructured nerve guide with longitudinal guidance channels
In vitro cell alignment obtained with a Schwann cell enriched microstructured nerve guide with longitudinal guidance channels
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DOI：
10.1016/j.biomaterials.2008.09.017
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发表时间：
2009-01-01
期刊：
影响因子：
14
通讯作者：
Pallua, Norbert
中科院分区：
文献类型：
--
作者：
Bozkurt, Ahmet;Deumens, Ronald;Pallua, Norbert
自体神经移植在修复周围神经损伤中的治疗益处尚未使用任何替代神经引导件达到。然而，并发症和有限的供体神经的可用性的问题迫切要求需要的生物人工神经引导，可以替代或补充自体神经移植物。越来越多的人认识到，最佳的神经引导包括支持外周轴突再生的物理和分子线索。现在，我们提出了一种基于胶原蛋白的微结构3D神经导向器，其中包含许多纵向导向通道，其尺寸类似于天然神经内膜管。此外，这些神经导管可以通过雪旺细胞（SC）的种植功能化。活的SC不仅粘附在神经引导件上，而且还在整个引导通道中迁移。特别重要的是观察到引导通道内的SC形成了让人联想到“Bungner带”的细胞柱，这是Wallerian变性期间周围神经再生的自然过程中的关键结构。因此，我们的结论是，我们的定向三维神经导（装饰与SC）与他们的物理和分子特性可能持有巨大的希望在周围神经损伤的修复，并作为未来的实验再生研究的基础。(c)2008爱思唯尔有限公司保留所有权利。
Therapeutic benefits of autologous nerve grafting in repair of peripheral nerve lesions have not been reached using any alternative nerve guide. Nevertheless, issues of co-morbidity and limited availability of donor nerves urgently ask for a need of bioartificial nerve guides which could either replace or complement autologous nerve grafts. It is increasingly appreciated that optimal nerve guides comprise both physical and molecular cues in support of peripheral axon regeneration. Now, we present a collagen-based microstructured 3D nerve guide containing numerous longitudinal guidance channels with dimensions resembling natural endoneurial tubes. Moreover, these nerve guides Could be functionalized by Schwann cell (SC) seeding. Viable SCs did not only adhere to the nerve guide, but also migrated throughout the guidance channels. Of particular importance was the observation that SCs within the guidance channels formed cellular columns reminiscent of "Bands of Bungner", which are crucial structures in the natural process of peripheral nerve regeneration during the Wallerian degeneration. We, therefore, conclude that our orientated 3D nerve guides (decorated with SCs) with their physical and molecular properties may hold great promise in the repair of peripheral nerve lesion and serve as a basis for future experimental regeneration studies. (c) 2008 Elsevier Ltd. All rights reserved.