Physiology and genotyping of adaptive and sensitive oil palm progenies under unwatered stress condition
Physiology and genotyping of adaptive and sensitive oil palm progenies under unwatered stress condition
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未浇水胁迫条件下适应性和敏感油棕后代的生理学和基因分型
DOI：
10.1088/1755-1315/293/1/012012
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
T. Liwang
中科院分区：
文献类型：
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作者：
D. Yono;E. Purwanti;A. Sahara;Y. A. Nugroho;Z. A. Tanjung;R. Aditama;C. Dewi;A. E. Sihotang;C. Utomo;T. Liwang
由于气候变化，油棕种植的干旱面积增加，导致育种者培育出耐旱的油棕。需要对遗传材料的生理和遗传特性进行表征。对两种不同的后代进行了分析，这两种后代来自坦桑尼亚，用于更适应缺水胁迫，尼日利亚来源用于更敏感。观察了树液流量、水势、土壤含水量、温室温度和相对湿度、气孔密度、气孔孔径和脯氨酸含量等生理相关变量。与尼日利亚品种相比，坦桑尼亚品种的液流更稳定，检测到的水势更长，气孔关闭适应性更强，脯氨酸含量更低。两种材料的基因组比较采用双消化限制性内切相关DNA进行，捕获了299 294个snp。研究发现，9.3%的snp在后代之间有一个频率较小的不同等位基因。在167个基因中，有0.7%的snp被定位和分布。在与细胞间水分转运相关的水通道蛋白NIP1-1基因中发现了两个SNP标记。一个SNP标记位于谷氨酸受体2.7样基因上，该基因与谷氨酸释放和脯氨酸生物合成有关。剩余的snp将被进一步分析。
Increasing of dry areas for Oil Palm plantation due to climate change led the breeders to develop drought stress, tolerant palm. Characterization of genetic materials for their physiological and genetic properties is required. Two distinct progenies derived from Tanzanian origin used for more adaptive to unwatered stress, and Nigerian origin used for more sensitive, were subjected for analysis. Physiological related variables were observed for sap flow, water potential, soil water content, greenhouse temperature and relative humidity, stomatal density, stomatal pore width, and proline content. The Tanzanian origin has more stable in sap flow, longer water potential detected, more adaptive in stomatal closure, and a lower in proline content than Nigerian origins. The genome comparison of both materials was performed by double digest Restriction Associated DNA and captured 299 294 SNPs. It found 9.3 % of those SNPs that were having a different allele with a minor frequency between progenies. And, 0.7 % from filtered SNPs were located and distributed in 167 genes. Two SNP markers were located in aquaporin NIP1-1 gene that related to water translocation between cells. One SNP marker was located in glutamate receptor 2.7-like gene that related to glutamate release and proline biosynthesis. The remaining SNPs will be in further analyzed.