Dynamic properties of round window membrane in guinea pig otitis media model measured with electromagnetic stimulation.
Dynamic properties of round window membrane in guinea pig otitis media model measured with electromagnetic stimulation.
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DOI：
10.1016/j.heares.2013.01.001
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发表时间：
2013-07
期刊：
影响因子：
2.8
通讯作者：
Zhang, Xiangming
中科院分区：
文献类型：
--
作者：
Gan, Rong Z.;Nakmali, Don;Zhang, Xiangming
圆窗是中耳进入耳蜗的两个开口之一，在听力中发挥着重要作用，并且已知在中耳炎期间会发生结构改变。然而，目前还没有发表的研究系统地描述伴随细菌性中耳炎的圆窗膜（RWM）生物力学特性的变化。在这里，我们描述了正常豚鼠和急性中耳炎（AOM）豚鼠之间RWM动态特性的显着变化，这些变化可以通过电磁力刺激和激光多普勒测振（LDV）测量来检测。通过将肺炎链球菌经球泡注射到中耳来诱导AOM，并在攻击三天后制备RWM标本。由线圈-磁铁耦合引起的 RWM 的振动通过 LDV 在 0.2-40 kHz 的频率范围内进行测量。然后在有限元模型中对实验进行模拟，并使用反问题求解方法确定频域中的复模量和时域中的松弛模量。 18 只耳朵（9 只对照耳朵和 9 只 AOM 耳朵）的结果表明，患有 AOM 的耳朵的 RWM 的储能模量和损耗模量均显着低于未感染耳朵的 RWM。 AOM耳的储能模量平均降低1.5至2.2 MPa，损耗模量平均降低0.025至0.48 MPa。我们的研究结果表明，由于 AOM 耳朵发生形态变化，中耳感染主要影响 RWM 的僵硬。我们还得出结论，当中耳植入式设备选择反向驱动时，用于评估 RWM 功能的线圈-磁体耦合方法可能会提供一种有价值的新方法来表征 RWM 的机械响应。
The round window, one of two openings into the cochlea from the middle ear, plays an important role in hearing and is known to be structurally altered during otitis media. However, there have been no published studies systematically describing the changes in biomechanical properties of the round window membrane (RWM) that accompany bacterial otitis media. Here we describe the occurrence of significant changes in the dynamic properties of the RWM between normal guinea pigs and those with acute otitis media (AOM) that are detectable by electromagnetic force stimulation and laser Doppler vibrometry (LDV) measurements. AOM was induced by transbullar injection of streptococcus pneumoniae into the middle ear, and RWM specimens were prepared three days after challenge. Vibration of the RWM induced by coil-magnet coupling was measured by LDV over frequencies of 0.2–40 kHz. The experiment was then simulated in a finite element model, and the inverse-problem solving method was used to determine the complex modulus in the frequency domain and the relaxation modulus in the time domain. Results from 18 ears (9 control ears and 9 AOM ears) established that both the storage modulus and loss modulus of the RWM from ears with AOM were significantly lower than those of RWM from uninfected ears. The average decrease of the storage modulus in AOM ears ranged from 1.5 to 2.2 MPa and the average decrease of the loss modulus was 0.025 to 0.48 MPa. Our findings suggest that middle ear infection primarily affects the stiffness of the RWM due to the morphological changes that occur in AOM ears. We also conclude that the coil-magnet coupling method for assessment of RWM function may provide a valuable new approach to characterizing the mechanical response of the RWM when reverse driving is selected for middle ear implantable devices.
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影响因子：
1.4
作者：
Colletti, Vittorio;Carner, Marco;Colletti, Liliana
通讯作者：
Colletti, Liliana
影响因子：
2.1
作者：
Beltrame, Achille M.;Martini, Alessandro;Streitberger, Christian
通讯作者：
Streitberger, Christian
影响因子：
3.4
作者：
Bausch, AR;Möller, W;Sackmann, E
通讯作者：
Sackmann, E
影响因子：
1.4
作者：
GOYCOOLEA, MV
通讯作者：
GOYCOOLEA, MV
影响因子：
2.7
作者：
Colletti, Vittorio;Soli, Sigfrid D.;Colletti, L.
通讯作者：
Colletti, L.