ROS scavenging effects of organic extract of diesel exhaust particles on human neutrophil granulocytes and rat alveolar macrophages
ROS scavenging effects of organic extract of diesel exhaust particles on human neutrophil granulocytes and rat alveolar macrophages
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DOI：
10.1016/j.tox.2006.11.057
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发表时间：
2007-02-12
期刊：
影响因子：
4.5
通讯作者：
Fonnum, F.
中科院分区：
文献类型：
--
作者：
Aam, Berit Bjugan;Fonnum, F.
柴油废气颗粒物是环境空气污染的主要成分，与呼吸系统和心血管疾病以及肺癌有关。这些颗粒的有机部分是异质性和复杂性的，似乎是许多不利影响的原因。ROS的形成增加往往与不良影响有关。因此，我们研究了柴油车尾气颗粒物有机提取物对体外培养的人中性粒细胞和大鼠肺泡巨噬细胞中ROS状态的影响。用DCFH-DA、光敏素和鲁米诺三种不同的方法研究了ROS的形成。DCFH-DA法测ROS时，有机提取物均能增加ROS的含量，而PMA刺激的细胞中ROS含量在三种测定方法中均有降低。在无细胞体系中，进一步研究了受影响的ROS的特性。无细胞实验证实，该提取物对超氧阴离子、次氯酸盐有清除作用，对过氧亚硝酸根也有一定的清除作用，但对羟基自由基和一氧化氮无清除作用。ROS参与了细胞内的信号通路以及对入侵微生物的防御，并讨论了细胞内氧化还原状态的干扰可能产生的影响。(C)2006爱思唯尔爱尔兰有限公司。保留所有权利。
Diesel exhaust particles are major constituents of ambient air pollution, and are associated with respiratory and cardiovascular diseases and lung cancer. The organic part of the particles is heterogenic and complex, and seems to be responsible for many of the adverse effects.Increased formation of ROS is often connected to the adverse effects. We have therefore investigated the effect of an organic extract of diesel exhaust particles on the reactive oxygen species (ROS) status in human neutrophil granulocytes and rat alveolar macrophages in vitro. ROS formation were studied by three different assays namely the use of DCFH-DA, lucigenin and luminol. The organic extract increased ROS assayed with DCFH-DA, but it decreased the amount of ROS in cells stimulated by PMA in all three assays. The identities of the ROS affected were further studied in cell free systems. The cell free studies confirmed that the extract had scavenging effects against superoxide, hypochlorite and to a smaller extent against peroxynitrite, but not against the hydroxyl radical and nitric oxide. ROS take part in the intracellular signalling pathways as well as in the defence against invading microorganisms, and the possible effects of interference of the redox status in the cells are discussed. (c) 2006 Elsevier Ireland Ltd. All rights reserved.