Antimicrobial resistance and its genetic determinants in aeromonads isolated in ornamental (koi) carp (Cyprinus carpio koi) and common carp (Cyprinus carpio)
Antimicrobial resistance and its genetic determinants in aeromonads isolated in ornamental (koi) carp (Cyprinus carpio koi) and common carp (Cyprinus carpio)
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DOI：
10.1016/j.vetmic.2009.10.001
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发表时间：
2010-05-19
影响因子：
3.3
通讯作者：
Vesely, Tomas
中科院分区：
文献类型：
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作者：
Cizek, Alois;Dolejska, Monika;Vesely, Tomas
本研究的目的是评估气单胞菌属的抗菌敏感性。从来自随机选择的养殖场的鲤鱼和锦鲤中分离出来。使用标准琼脂稀释药敏试验测试分离株对 8 种抗菌剂的敏感性。在所有分离株中，使用 PCR 检测 tet(A-E) 基因、整合酶基因和基因盒的存在。从锦鲤采样的总共 72 株运动气单胞菌中，36 株（50%）对土霉素耐药，18 株（25%）对环丙沙星耐药，5 株（7%）对氯霉素耐药，5 株（7%）对氟苯尼考耐药，11 株（15%）对甲氧苄啶耐药。从鲤鱼中采集的 49 株运动气单胞菌中，20 株（41%）对土霉素耐药，3 株（6%）对氯霉素耐药，3 株（6%）对氟苯尼考耐药。锦鲤气单胞菌对环丙沙星的耐药性显着较高(P=0.00024)。仅在这些分离株中检测到 1 类整合子的存在 (P=0.00024)。在 40% (48/121) 的分离株中检测到 Tet 基因，其中 tet(E) 是最主要的。我们的结果表明，从锦鲤和鲤鱼中收集的耐药菌株的发生率存在显着差异（P=0.00042）。这种差异可归因于消费养鱼场与观赏养鱼场制定的不同抗生素政策。在两种类型的养鱼场之间进行技术交叉的情况下，应考虑耐药细菌传播并将感染传播给人类的潜在风险。 (C) 2009 Elsevier B.V. 保留所有权利。
The aim of this study was to evaluate antimicrobial susceptibility of Aeromonas spp. isolates from common carp and koi carp coming from randomly chosen farms. The isolates were tested for susceptibility to 8 antimicrobial agents using the standard agar dilution susceptibility test. In all isolates, PCR was used to detect the presence of tet(A-E) genes, integrase genes, and gene cassettes. From the total 72 isolates of motile aeromonads sampled from koi carp, 36 isolates (50%) were resistant to oxytetracycline, 18 (25%) to ciprofloxacin, 5 (7%) to chloramphenicol, 5 (7%) to florfenicol, and 11 (15%) to trimethoprim. Among 49 isolates of motile aeromonads collected from common carp, 20 (41%) were resistant to oxytetracycline, 3 (6%) to chloramphenicol, and 3 (6%) to florfenicol. The resistance of aeromonads isolated from koi carp was significantly higher to ciprofloxacin (P=0.00024). The presence of class 1 integrons was detected in these isolates only (P=0.00024). Tet genes were detected in 40% (48/121) of isolates, with tet(E) being the most dominant. Our results demonstrated a significant difference in the incidence of resistant isolates collected from koi carp and common carp (P=0.00042). This difference can be ascribed to a distinct antibiotic policy established on consumer fish farms versus ornamental fish farms. The potential risk for resistant bacteria to spread and transmit infection to humans should be considered in cases of technological crossover between the two types of fish farms. (C) 2009 Elsevier B.V. All rights reserved.