Disposable Polyurethane Nanospiked Gold Electrode-Based Label-Free Electrochemical Immunosensor for Clostridium difficile
Disposable Polyurethane Nanospiked Gold Electrode-Based Label-Free Electrochemical Immunosensor for Clostridium difficile
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DOI：
10.1021/acsanm.9b02001
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发表时间：
2020-01-01
影响因子：
5.9
通讯作者：
Zhou, H. Susan
中科院分区：
文献类型：
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作者：
Cui, Feiyun;Zhou, Zhiru;Zhou, H. Susan
研制了一种用于艰难梭菌（C. difficile）毒素B检测的聚氨酯（PU）纳米尖金电极无标记电化学免疫传感器。与纳米材料修饰电极不同，PU纳米尖金电极采用软光刻技术直接制备。利用扫描电镜（SEM）对纳米尖刺电极的形貌进行了表征，并利用循环伏安法（CV）和差分脉冲伏安法（DPV）对其良好的电化学性能进行了表征。然后，通过在电极表面固定抗毒素B单域抗体作为受体，制备了基于PU纳米尖金电极的免疫传感器。采用DPV检测技术检测艰难梭菌毒素B。结果表明，该免疫传感器具有良好的特异性、重复性和稳定性。即使在无标签的情况下，毒素B的检出限为0.5 pg/mL。在1 ~ 130 pg/mL浓度范围内，δ I = -1.216[C] - 41.62呈线性回归方程；相关系数R-2为0.9839。与平面PU电极免疫传感器相比，PU纳米尖金电极免疫传感器的检测信号放大了约6倍。基于PU纳米尖金电极的免疫传感器具有成本低、加工简单等优点，可作为一次性电化学传感器用于毒素B的快速检测。
A polyurethane (PU) nanospiked gold electrode-based label-free electrochemical immunosensor for Clostridium difficile (C. difficile) toxin B detection was developed. Different from nanomaterial-modified electrodes, the PU nanospiked gold electrode was fabricated by soft lithography directly. The morphology of the nanospiked electrode was characterized by scanning electron microscope (SEM), and its good electrochemical performance was demonstrated by cyclic voltammetry (CV) as well as differential pulse voltammetry (DPV). Then, the PU nanospiked gold electrode-based immunosensor was developed by fixing antitoxin B single domain antibody on the electrode surface as the receptor. DPV was used as a detection technology for C. difficile toxin B detection. It revealed that the immunosensor has good specificity, repeatability, and stability. Even in a label-free style, the limit of detection for toxin B was 0.5 pg/mL. In the concentration range of 1-130 pg/mL, the linear regression equation Delta I = -1.216[C] - 41.62 was found; the correlation coefficient R-2 was 0.9839. Compared with the flat PU electrode-based immunosensor, the detection signal of the PU nanospiked gold electrode-based immunosensor was amplified about 6 times. Benefiting from its low cost and simple processing, the PU nanospiked gold electrode-based immunosensor can be used as a disposable electrochemical sensor for toxin B rapid detection.