Systematic and Cell Type-Specific Telomere Length Changes in Subsets of Lymphocytes
Systematic and Cell Type-Specific Telomere Length Changes in Subsets of Lymphocytes
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DOI：
10.1155/2016/5371050
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发表时间：
2016-01-01
影响因子：
4.1
通讯作者：
Blackburn, Elizabeth H.
中科院分区：
文献类型：
--
作者：
Lin, Jue;Cheon, Joshua;Blackburn, Elizabeth H.
端粒是线性染色体末端的保护性 DNA-蛋白质复合物，对于基因组稳定性非常重要。白细胞或外周血单核细胞 (PBMC) 端粒长度是人类衰老的潜在生物标志物，综合了遗传、环境和生活方式因素，并与死亡率和重大疾病风险相关。然而，只有有限数量的研究检查了端粒长度的纵向变化，并且很少报告有关分选循环免疫细胞的数据。我们在一组绝经前女性中横向和纵向检查了 CD4+、CD8+CD28+ 和 CD8+CD28- T 细胞、B 细胞和 PBMC 的平均端粒长度 (TL)。我们报告说，同一参与者的这三种 T 细胞类型在 18 个月内的 TL 变化是相关的。此外，PBMC TL 变化也与所有三种 T 细胞类型和 B 细胞的变化相关。 B 细胞的缩短率明显高于三种 T 细胞类型。尽管 CD8+CD28- 细胞的 TL 最短，但与 CD8+CD28+ T 细胞相比，CD8+CD28- 细胞的损耗速度明显更快。这些结果表明，系统协调的、细胞类型特异性的因子和途径反应有助于端粒长度调节。
Telomeres, the protective DNA-protein complexes at the ends of linear chromosomes, are important for genome stability. Leukocyte or peripheral blood mononuclear cell (PBMC) telomere length is a potential biomarker for human aging that integrates genetic, environmental, and lifestyle factors and is associated with mortality and risks for major diseases. However, only a limited number of studies have examined longitudinal changes of telomere length and few have reported data on sorted circulating immune cells. We examined the average telomere length (TL) in CD4+, CD8+CD28+, and CD8+CD28- T cells, B cells, and PBMCs, cross-sectionally and longitudinally, in a cohort of premenopausal women. We report that TL changes over 18 months were correlated among these three T cell types within the same participant. Additionally, PBMC TL change was also correlated with those of all three T cell types, and B cells. The rate of shortening for B cells was significantly greater than for the three T cell types. CD8+CD28- cells, despite having the shortest TL, showed significantly more rapid attrition when compared to CD8+CD28+ T cells. These results suggest systematically coordinated, yet cell type-specific responses to factors and pathways contribute to telomere length regulation.