Characterization of thymus atrophy in piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus
Characterization of thymus atrophy in piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus
复制标题
高致病性猪繁殖与呼吸综合征病毒感染仔猪胸腺萎缩的特征
DOI：
10.1016/j.vetmic.2012.05.040
复制
发表时间：
2012-12-07
影响因子：
3.3
通讯作者：
Cai, Xuehui
中科院分区：
文献类型：
--
作者：
He, Yuli;Wang, Gang;Cai, Xuehui
本实验室前期研究表明，高致病性猪繁殖与呼吸综合征病毒（HP-PRRSV）感染仔猪后，胸腺明显萎缩。本研究旨在进一步探讨HP-PRRSV和PRRSV感染仔猪后胸腺的病变及胸腺T细胞亚群的变化。观察胸腺/体重比、胸腺病理变化、胸腺病毒载量、胸腺细胞凋亡及超微结构。结果表明，HP-PRRSV感染仔猪的胸腺/体重比显著低于PRRSV感染或对照仔猪，胸腺病变的特征是胸腺细胞严重耗竭。接种HP-PRRSV后7 d，发生凋亡的胸腺细胞数比PRRSV感染组增加约40倍。HP-PRRSV感染仔猪的双阳性胸腺细胞比任一单阳性亚群受到更大程度的抑制，但在PRRSV感染仔猪中未观察到类似的结果。提示HP-PRRSV诱导的大量细胞凋亡可能导致胸腺严重萎缩和胸腺细胞耗竭。(C)2012爱思唯尔有限公司版权所有。
Our previous study has demonstrated that piglets infected with highly pathogenic PRRSV (HP-PRRSV) induced significant thymus atrophy. The aim of this study was to further investigate the lesions in thymus of piglets infected with HP-PRRSV or PRRSV and the changes of thymic T cell populations. The lesions were evaluated for the thymus/body weight ratios, pathological changes and virus load in thymus, apoptosis and ultrastructure of thymocytes. The result showed that thymus/body weight ratios of HP-PRRSV-infected piglets were significantly reduced compared to PRRSV-infected or control piglets, and thymic lesions were characterized by severe cortical depletion of thymocytes. The number of thymocytes undergoing apoptosis was increased approximately forty-fold in piglets infected with HP-PRRSV than that of PRRSV on 7 days post-inoculation (DPI). Double-positive thymocytes of piglets infected with HP-PRRSV were suppressed to a greater degree than either single positive subpopulation, but similar results were not observed in piglets infected with PRRSV. These results suggested that HP-PRRSV induced abundant apoptosis might result in severe thymus atrophy and the depletion of thymocytes. (C) 2012 Elsevier B.V. All rights reserved.