p53 antibodies in the sera of lung cancer patients: Comparison with p53 mutation in the tumour tissue
p53 antibodies in the sera of lung cancer patients: Comparison with p53 mutation in the tumour tissue
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肺癌患者血清中p53抗体：与肿瘤组织中p53突变的比较
DOI：
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发表时间：
1995
影响因子：
6.4
通讯作者：
H. Vainio
中科院分区：
文献类型：
--
作者：
C. Wild;M. Ridanpaa;S. Anttila;R. Lubin;T. Soussi;K. Husgafvel‐Pursiainen;H. Vainio
本研究检查了血清自身抗体对p53蛋白的敏感性和特异性，作为肺癌病例中p53基因改变或蛋白积累的非侵入性标志物。建立了一种灵敏的检测血清p53抗体的ELISA方法，并用于检测186例因疑似肺癌而接受肺手术的患者的血清。ELISA中的靶抗原是野生型p53蛋白和覆盖蛋白的N-和C-末端部分的5种肽。16例血清p53抗体在两种ELISA中均呈阳性，且均在136例确诊的原发性肺癌患者中。在50例其他肺部疾病患者中，无一人有p53抗体。在92例癌症患者的p53基因外显子5至8的突变进行了检查变性梯度凝胶电泳和PCR产物的直接测序。47例肿瘤有p53突变，其中7例（15.2%）p53抗体阳性。2例患者有血清抗体，但在外显子5至8没有检测到突变。鳞状细胞癌患者p53突变和血清抗体的频率高于腺癌。在32例患者中观察到p53蛋白在肿瘤组织中积聚，但仅5例p53抗体阳性。总之，血清p53抗体仅在一部分肺癌病例中检测到，但大多数与肿瘤中可检测到的p53突变特异性相关。© 1995 Wiley利斯公司
This study examined the sensitivity and specificity of serum auto‐antibodies to p53 protein as a non‐invasive marker of p53 genetic alterations or protein accumulation in lung cancer cases. A sensitive ELISA to detect serum p53 antibodies was developed and used to examine sera from 186 patients undergoing pulmonary surgery for a suspected lung cancer. Target antigens in ELISA were wild‐type p53 protein and 5 peptides covering the N‐ and C‐terminal parts of the protein. Sixteen sera were positive for serum p53 antibodies in both ELISAs and all were among the 136 patients with confirmed primary lung carcinoma. Of 50 patients with other pulmonary diseases, none had p53 antibodies. In 92 cancer patients exons 5 to 8 of the p53 gene were examined for mutations by denaturing gradient gel electrophoresis and direct sequencing of PCR products. Forty‐seven tumours had a p53 mutation and 7 (15.2%) of these were positive for p53 antibodies. Two patients had serum antibodies but no detectable mutation in exons 5 to 8. Frequencies of p53 mutations and serum antibodies were higher in squamous cell carcinoma patients than in adenocarcinoma. Accumulation of p53 protein in tumour tissue was observed in 32 patients, but only 5 were positive for p53 antibodies. In conclusion, serum p53 antibodies were detected only in a proportion of lung cancer cases, but the majority were specifically associated with a detectable p53 mutation in the tumour. © 1995 Wiley‐Liss, Inc.
影响因子：
56.9
作者：
HOLLSTEIN, M;SIDRANSKY, D;HARRIS, CC
通讯作者：
HARRIS, CC
DOI：
10.1073/pnas.89.8.3439
发表时间：
1992-04-15
影响因子：
11.1
作者：
DAVIDOFF, AM;IGLEHART, JD;MARKS, JR
通讯作者：
MARKS, JR