Antiherpes simplex virus type 2 activity of casuarinin from the bark of Terminalia arjuna Linn
Antiherpes simplex virus type 2 activity of casuarinin from the bark of Terminalia arjuna Linn
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DOI：
10.1016/s0166-3542(02)00077-3
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发表时间：
2002-09-01
影响因子：
7.6
通讯作者：
Lin, TC
中科院分区：
文献类型：
--
作者：
Cheng, HY;Lin, CC;Lin, TC
木麻黄素(Casuarinin)是从金鸡儿(Terminalia Arjua Linn.)树皮中分离得到的一种水解性单宁。(菊科)进行了体外抗单纯疱疹病毒(HSV-2)活性的研究。结果表明，木麻黄素对XTT和空斑减少试验的IC50分别为3.6±0.9和1.5±0.2微米。细胞生长的半数细胞毒浓度(CC50)为89+/-1um。因此，在XTT和空斑减少试验中，木麻黄素的选择性指数(SI)(CC50/IC50)分别为25和59。木麻黄素在感染后12h仍有抗病毒活性。在黏附试验中，木麻黄素被证明可以阻止HSV-2与细胞的黏附。此外，木麻黄素还显示出抑制病毒穿透的活性。有趣的是，木麻黄素在25微米的浓度下具有病毒杀伤力，可将病毒滴度降低高达100,000倍。本研究得出结论：木麻黄素具有抗疱疹病毒活性，抑制病毒附着和穿透，并干扰感染的晚期事件(S)。(C)2002 Elsevier Science B.V.保留所有权利。
Casuarinin, a hydrolyzable tannin isolated from the bark of Terminalia arjuna Linn. (Combretaceae), was investigated for its antiviral activity on herpes simplex type 2 (HSV-2) in vitro. Results showed that the IC50 Of casuarinin in XTT and plaque reduction assays were 3.6 +/- 0.9 and 1.5 +/- 0.2 muM, respectively. The 50% cytotoxic concentration for cell growth (CC50) was 89 +/- 1 muM. Thus, the selectivity index (SI) (ratio Of CC50 to IC50) of casuarinin was 25 and 59 for XTT and plaque reduction assays, respectively. Casuarinin continued to exhibit antiviral activity even added 12 h after infection. During the attachment assay, casuarinin was shown to prevent the attachment of HSV-2 to cells. Furthermore, casuarinin also exhibited an activity in inhibiting the viral penetration. Interestingly, casuarinin was virucidal at a concentration of 25 muM, reducing viral titers up to 100,000-fold. This study concludes that casuarinin possesses anti-herpesvirus activity in inhibiting viral attachment and penetration, and also disturbing the late event(s) of infection. (C) 2002 Elsevier Science B.V. All rights reserved.