FROZEN STORAGE OF RAM SEMEN .2. CAUSES OF LOW FERTILITY AFTER CERVICAL INSEMINATION AND METHODS OF IMPROVEMENT
FROZEN STORAGE OF RAM SEMEN .2. CAUSES OF LOW FERTILITY AFTER CERVICAL INSEMINATION AND METHODS OF IMPROVEMENT
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DOI：
10.1016/0378-4320(94)01328-j
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发表时间：
1995-03-01
影响因子：
2.2
通讯作者：
MAXWELL, WMC
中科院分区：
文献类型：
--
作者：
SALAMON, S;MAXWELL, WMC
研究到1993年进行了审查。绵羊精液的冷冻和解冻会对精子的超微结构、生化和功能造成很大程度的损伤。这些变化伴随着精子在女性生殖道中的活力降低，运输受损和活力降低，以及宫颈授精后生育力降低。用于提高宫颈授精后生育力的方法包括增加授精液中精子的浓度，用激素处理母羊或向精液中添加激素，双重和深度宫颈授精。最有效的方法是增加冻融精液在宫颈管内的沉积深度。最近发展的经宫颈授精技术实现了宫颈深部授精，甚至精液在子宫沉积。然而，目前只有通过腹腔镜宫腔内授精才能获得满意和可靠的产羔结果。
Research up to 1993 is reviewed. Freezing and thawing of ram semen causes ultrastructural, biochemical and functional damage to a significant proportion of spermatozoa. These changes are accompanied by a reduction in motility, impaired transport and decreased viability of spermatozoa in the female genital tract, and reduced fertility after cervical insemination.Methods used to improve the fertility after cervical insemination include increased concentration of spermatozoa in the inseminate, treatment of ewes with or addition to the semen of hormones, double and deep cervical inseminations. The most effective method is to increase the depth of deposition of frozen-thawed semen into the cervical canal. Recently developed transcervical insemination techniques achieve deep cervical insemination or even uterine deposition of semen. However, at present satisfactory and reliable lambing results are only obtainable by using intrauterine insemination by laparoscopy.