Genetic and phenotypic characterization of the newly described insect flavivirus, Kamiti River virus
Genetic and phenotypic characterization of the newly described insect flavivirus, Kamiti River virus
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DOI：
10.1007/s00705-003-0019-7
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发表时间：
2003-06-01
影响因子：
2.7
通讯作者：
Miller, BR
中科院分区：
文献类型：
--
作者：
Crabtree, MB;Sang, RC;Miller, BR
我们在Sang等人的随附论文中描述了，([57]，Arch Virol 2003：1085-1093），分离和鉴定了一种新的黄病毒，Kamiti River病毒（KRV）。macintoshi蚊子作为幼虫和蛹从肯尼亚中央省的洪水泛滥的dambos收集。在已知的黄病毒中，KRV显示出与细胞融合剂病毒（CFAV）最相似，但在遗传和表型上不同。KRV暂时被鉴定为一种仅在昆虫中存在的黄病毒，其不能在脊椎动物细胞或小鼠中复制。我们在此报告了KRV在细胞培养物中生长的进一步表征，并将其与CFAV进行了比较;尽管生长动力学相似，但KRV不会引起CFAV感染所特有的细胞融合。发现KRV基因组长度为11，375个核苷酸，含有编码10种病毒蛋白的单个开放阅读框。鉴定了可能的多蛋白裂解位点，其与CFAV的那些最相似，并且与其他黄病毒的那些相当。与其他黄病毒的序列同一性较低;与CFAV的最大同一性。5'和3'非编码区（NCR）的可能末端二级结构与其他黄病毒的预测相似。尽管CFAV是在实验室中从昆虫细胞中分离出来的，但KRV的分离证明了自然界中存在一种仅限昆虫的黄病毒，并提出了关于该病毒与其他蚊媒病毒在感受态载体群体中潜在相互作用的问题。此外，这种病毒将是未来研究中确定与黄病毒宿主特异性相关的标志物的重要工具。
We have described in the accompanying paper by Sang, et al., ([57], Arch Virol 2003: 1085-1093) the isolation and identification of a new flavivirus, Kamiti River virus (KRV), from Ae. macintoshi mosquitoes that were collected as larvae and pupae from flooded dambos in Central Province, Kenya. Among known flaviviruses, KRV was shown to be most similar to, but genetically and phenotypically distinct from, Cell fusing agent virus (CFAV). KRV was provisionally identified as an insect-only flavivirus that fails to replicate in vertebrate cells or in mice. We report here the further characterization of KRV Growth in cell culture was compared to that of CFAV; although growth kinetics were similar, KRV did not cause the cell fusion that is characteristic of CFAV infection. The KRV genome was found to be 11,375 nucleotides in length, containing a single open reading frame encoding 10 viral proteins. Likely polyprotein cleavage sites were identified, which were most similar to those of CFAV and were comparable to those of other flaviviruses. Sequence identity with other flaviviruses was low; maximum identity was with CFAV Possible terminal secondary structures for the 5' and 3' non-coding regions (NCR) were similar to those predicted for other flaviviruses. Whereas CFAV was isolated from insect cells in the laboratory, the isolation of KRV demonstrates the presence of an insect-only favivirus in nature and raises questions regarding potential interactions between this virus and other mosquito-borne viruses in competent vector populations. Additionally, this virus will be an important tool in future studies to determine markers associated with flavivirus host specificity.