Analysis of anisotropic diamagnetic susceptibility of a bull sperm
Analysis of anisotropic diamagnetic susceptibility of a bull sperm
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DOI：
10.1002/bem.10109
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发表时间：
2003-07-01
影响因子：
1.9
通讯作者：
Higashi, T
中科院分区：
文献类型：
--
作者：
Emura, R;Takeuchi, T;Higashi, T
公牛精子和草履虫纤毛暴露于均匀的静态磁场，观察其磁取向和测量其各向异性抗磁化率（Deltachi）为每个。制备的样品是完整的公牛精子、公牛精子平头和草履虫纤毛，因为无法制备完美状态的公牛精子尾部。整个公牛精子和公牛精子头的方向垂直于磁场（最大1.7特斯拉），而草履虫纤毛则平行于磁场（最大8特斯拉）。对整个公牛精子、公牛精子头和草履虫纤毛进行光度学研究，以获得每种精子的Deltachi，估计分别为1 x 10（-19）、3 x 10（-19）和2 x 10（-20）J/T-2。根据草履虫纤毛Deltachi的测量值估算了精子鞭毛Deltachi，这与整个精子和精子头部Deltachi之间的差异吻合得很好。此外，Deltachi的这种差异也几乎与从微管蛋白的Deltachi计算的Deltachi值一致。如果解决了磁对生物系统的影响，并将磁取向与之相关联，则Deltachi将成为该效应的定量指标。(C)2003 Wiley-Liss，Inc.
Bull sperm and paramecium cilium were exposed to uniform static magnetic fields to observe their magnetic orientations and measure their anisotropic diamagnetic susceptibility (Deltachi) for each. The prepared samples were whole bull sperm, bull sperm flat heads, and paramecium cilia, because bull sperm tails in a perfect condition could not be prepared. The whole bull sperm and the bull sperm heads became oriented perpendicular to the magnetic fields (1.7 Tesla maximum), while the paramecium cilia became parallel to the magnetic fields (8 Tesla maximum). A whole bull sperm, a bull sperm head, and a paramecium cilium were photometrically studied to obtain Deltachi for each, which were estimated to be 1 x 10(-19), 3 x 10(-19), and 2 x 10(-20) J/T-2, respectively. Deltachi of a sperm flagellum was estimated from the measured value of Deltachi of the paramecium cilium, which agrees well with the difference between Deltachi of the whole sperm and the sperm head. Additionally, this difference of Deltachi almost coincides with the Deltachi values calculated from Deltachi of tubulin, as well. If the magnetic effect on biological systems is solved and the magnetic orientation correlates with it, Deltachi will become the quantitative index of the effect. (C) 2003 Wiley-Liss, Inc.