Non-protein amino acids in muscle and blood of marine and fresh water Crustacea.
Non-protein amino acids in muscle and blood of marine and fresh water Crustacea.
复制标题
海洋和淡水甲壳类动物肌肉和血液中的非蛋白质氨基酸。
DOI：
10.1016/s0021-9258(18)50943-1
复制
发表时间：
1951
影响因子：
4.8
通讯作者：
M. Florkin
中科院分区：
文献类型：
--
作者：
M. Camien;H. Sarlet;G. Duchateau;M. Florkin
分离白鹤腹部、河虾腹部和大螯、锯齿鲨和中华绒螯蟹腿部和大螯的肌肉样品，称重并放入沸水中煮5分钟。灭活蛋白水解酶，均质化并用钨酸处理。 Scrum 样品用钨酸处理，所有不含蛋白质的样品通过回流 24 小时进行水解。与 6NHC1。天冬氨酸、精氨酸、谷氨酸、甘氨酸、异亮氨酸、亮氨酸、赖氨酸、蛋氨酸。苯丙氨酸、缬氨酸、酪氨酸。测定了丙氨酸、组氨酸、脯氨酸和苏氨酸。白鹤、绒螯蟹和星虾血清中的氨基酸浓度远低于肌肉中的浓度，白鹤肌肉中胞外氨基酸的计算值为9.3 mg。与细胞外和细胞内氨基酸总量 3031.6 mg 相比，可以忽略不计。百分比。甘氨酸、脯氨酸、精氨酸和谷氨酸是甲壳动物肌肉中最丰富的氨基酸，它们的摩尔浓度似乎足以产生相当大的渗透压。海洋物种肌肉中细胞内氨基酸的浓度高于淡水物种。所提到的游离酸可能是调节甲壳动物肌肉渗透压的重要物质。-A．伊戈。
Muscle samples from abdomen ofHomarus vulgaris, abdomen and large chela ofAstacus fluviatilis, and legs and large chela ofMaio squinadoandEriocheir sinensiswere isolated, weighed and placed in boiling water for 5 min. to inactivate proteolytic enzymes, homogenized and treated with tungstic acid. Scrum samples were treated with tungstic acid and all the protein-free samples were hydrolysed by refluxing for 24 hr. with 6NHC1. Aspartic acid, arginine, glutamic acid, glycine, isoleucine, leucine, lysine, methionine. phenylalanine, valine, tryosine. alanine, histidine, proline and threonine were estimated.The concentrations of amino-acids in the blood serum ofHomarus, EriocheirandAstacuswere, much less than those in muscle, and the calculated value for extracellular amino-acids inHomarusmuscle, 9.3 mg. per cent., was negligible in comparison with the total of extracellular and intracellular amino-acids, 3031.6 mg. per cent. Glycine, proline, arginine, and glutamic acid were the most abundant amino-acids in crustacean muscle and their molar concentrations appear sufficient to produce considerable osmotic pressure. The concentrations of intracellular amino-acids in muscles of marine species were higher than in fresh water species. The free acids mentioned are probably important substances for the regulation of osmotic pressure in crustacean muscle.-A. Iggo.