Analysis of different pigmentation patterns in 'Mantianhong' (Pyrus pyrifolia Nakai) and 'Cascade' (Pyrus communis L.) under bagging treatment and postharvest UV-B/visible irradiation conditions
Analysis of different pigmentation patterns in 'Mantianhong' (Pyrus pyrifolia Nakai) and 'Cascade' (Pyrus communis L.) under bagging treatment and postharvest UV-B/visible irradiation conditions
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套袋处理和采后 UV-B/可见光照射条件下“满天红”(Pyrus perifolia Nakai) 和“Cascade”(Pyrus communis L.) 不同色素沉着模式的分析
DOI：
10.1016/j.scienta.2012.12.020
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发表时间：
2013-02-28
影响因子：
4.3
通讯作者：
Teng, Yuanwen
中科院分区：
文献类型：
--
作者：
Qian, Minjie;Zhang, Dong;Teng, Yuanwen
本研究对套袋和采后UV-B/可见光照射条件下的‘满天红’（Pyrus pyrifolia Nakai）和‘Cascade’（Pyrus communis L.）色素沉着模式进行了研究。结果表明，与“Cascade”几乎没有检测到花青素不同，“Mantianhong”在套袋处理和采后辐照下呈现出良好的红色。PAL和DFR活性随着花青素积累的增加而增加。PpCHS3、PpCHI3、pppufgt1和PyMYB10的表达在“满天红”中上调，而在“Cascade”中均未上调。这表明这四个基因可能是“级联”果皮颜色的限制因素。基因家族成员在果实变红过程中的表达模式也不同，表明不同基因家族成员在梨发育过程中可能具有不同的功能。本研究结果有助于了解红梨花青素积累的分子机制，可为开展标记辅助选择或梨花青素代谢工程提供依据。(C) 2012 Elsevier B.V.版权所有
The pigmentation patterns of 'Mantianhong' (Pyrus pyrifolia Nakai) and 'Cascade' (Pyrus communis L.) that develop under bagging and postharvest UV-B/visible irradiation conditions were evaluated in this study. The results showed that, unlike 'Cascade,' in which almost no anthocyanin was detected, 'Mantianhong' developed good red coloration under bagging treatment and postharvest irradiation. The activity of PAL and DFR increased during anthocyanin accumulation. The expression of PpCHS3, PpCHI3, PpUFGT1, and PyMYB10 was up-regulated in 'Mantianhong' but not in 'Cascade' during both treatments. This suggests that these four genes may be limiting factors for peel coloration in 'Cascade.' The expression patterns of gene family members were also different during fruit red coloration, suggesting that different gene family members may have distinct functions during pear development. The results obtained in this study are helpful in understanding the molecular mechanism of anthocyanin accumulation in red pears and can serve as the basis for the development of marker-assisted selection or for anthocyanin metabolic engineering of pears. (C) 2012 Elsevier B.V. All rights reserved.