Theoretical consideration of amplification strategies
Theoretical consideration of amplification strategies
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DOI：
10.1023/a:1020917223962
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发表时间：
2002-10-01
影响因子：
4.4
通讯作者：
Shaw, CA
中科院分区：
文献类型：
--
作者：
Shaw, CA
实验者希望对从小细胞群体甚至单个细胞中提取的样本进行大规模的表达分析。在所有现有的阵列技术下，需要对来源材料进行放大才能对如此小的样本进行分析。在这份报告中，我们考虑了线性和基于聚合酶链式反应的扩增非常不同的理论性质。提出了一种结合两种方法的优点的混合扩增策略。
Experimentalists would like to perform large-scale expression analysis on samples drawn from small cell populations and even single cells. Amplification of the source material is required to assay such small samples under all existing array technologies. In this report we consider the very different theoretical properties of linear and PCR-based amplification. A suggestion is made concerning a hybrid amplification strategy incorporating the advantages offered by both approaches.