IDENTIFICATION OF TOXS, A REGULATORY GENE WHOSE PRODUCT ENHANCES TOXR-MEDIATED ACTIVATION OF THE CHOLERA-TOXIN PROMOTER
IDENTIFICATION OF TOXS, A REGULATORY GENE WHOSE PRODUCT ENHANCES TOXR-MEDIATED ACTIVATION OF THE CHOLERA-TOXIN PROMOTER
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DOI：
10.1128/jb.171.3.1288-1293.1989
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发表时间：
1989-03-01
影响因子：
3.2
通讯作者：
MEKALANOS, JJ
中科院分区：
文献类型：
--
作者：
MILLER, VL;DIRITA, VJ;MEKALANOS, JJ
我们描述了霍乱弧菌 E Tor 菌株 E7946 的 toxS 基因的克隆。该基因位于 toxR 基因的下游，该基因编码霍乱弧菌中霍乱毒素 (ctx) 操纵子的转录激活子。我们发现 ToxS 与 ToxR 联合作用，激活大肠杆菌中 ctx 操纵子的表达。经典菌株 569B 的毒力减弱，但在体外合成高水平的霍乱毒素，在 toxR 基因下游删除了 1.2 KB DNA，从而去除了 toxS。我们提供的证据表明，toxS 是具有 toxR 的操纵子中的下游基因。
We describe the cloning of the toxS gene from Vibrio cholerae E Tor strain E7946. This gene lies downstream from the toxR gene, which encodes the transcriptional activator for the cholera toxin (ctx) operon in V. cholerae. We show that ToxS acts in conjunction with ToxR to activate expression of the ctx operon in Escherichia coli. The classical strain 569B, which is attenuated for virulence but which synthesizes high levels of cholera toxin in vitro, carries a deletion of 1.2 kilobase pairs of DNA, downstream from the toxR gene, which removes toxS. We present evidence that toxS is the downstream gene in an operon with toxR.