Isolation of mouse intrahepatic lymphocytes.
Isolation of mouse intrahepatic lymphocytes.
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DOI：
10.1002/0471142735.im0321s22
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发表时间：
2001-05-01
影响因子：
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通讯作者：
Crispe, I Nicholas
中科院分区：
文献类型：
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作者：
Crispe, I Nicholas
肝脏是一个独特的免疫环境，其中存在多种细胞类型。肝内淋巴细胞（IHL）的绝对数量和肝脏中细胞混合物的各种成分的频率受到小鼠的年龄和品系以及激素、细胞因子和病原体的存在的影响。肝脏的大部分由肝细胞组成。除了 IHL 之外，肝脏还含有巨噬细胞群、库普弗细胞和肝窦内皮细胞。这里描述的协议可用于消耗肝组织的肝细胞、内皮细胞和红细胞，留下主要由 IHL 组成的细胞悬浮液，其中主要污染物是库普弗细胞（可以通过粘附塑料去除）。该单位提供了两种可用于隔离国际人道法的方案。一种方法可用于从多个肝脏中并行分离 IHL，而更精细的替代方案可在每个肝脏中产生更多细胞，但更适合用于从单个肝脏中回收 IHL。
The liver is a distinctive immunological environment in which are found a wide variety of cell types. The absolute number of intrahepatic lymphocytes (IHL) and the frequency of the various components of the cell mixture in the liver are influenced by the age and strain of the mouse, as well as by the presence of hormones, cytokines, and pathogens. The bulk of the liver consists of hepatocytes. In addition to IHL, the liver also contains a macrophage population, the Kupffer cells, and sinusoidal endothelial cells. The protocols described here can be used to deplete the liver tissue of hepatocytes, endothelial cells, and red blood cells, leaving a cell suspension consisting mainly of IHL in which the major contaminant is Kupffer cells (which can be removed by adherence to plastic). This unit provides two protocols that may be used to isolate IHL. One can be used to isolate IHL from multiple livers in parallel, whereas the more elaborate alternate protocol yields more cells per liver but is more appropriately used to recover the IHL from a single liver.