VdOGDH is involved in energy metabolism and required for virulence of Verticillium dahliae
VdOGDH is involved in energy metabolism and required for virulence of Verticillium dahliae
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VdOGDH 参与能量代谢，是大丽黄萎病菌毒力所必需的
DOI：
10.1007/s00294-019-01025-2
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发表时间：
2020-04-01
期刊：
影响因子：
2.5
通讯作者：
Cheng, Hongmei
中科院分区：
文献类型：
--
作者：
Li, Xiaokang;Su, Xiaofeng;Cheng, Hongmei
大丽轮枝菌（Verticillium dahliae）是一种土传真菌，能侵入植物的维管组织，引起植物黄萎病。酶α-酮戊二酸脱氢酶（OGDH）催化三羧酸循环（TCA）中α-酮戊二酸的氧化，对于真菌中的能量代谢至关重要。在此，我们鉴定了大丽弧菌中的OGDH基因（VdOGDH，VDAG_10018），并通过产生基因缺失突变体（Delta VdOGDH）和互补突变体（Delta VdOGDH-C）来研究其在毒力中的功能。当Delta VdOGDH突变体补充不同的碳源时，Czapek Dox培养基上的营养生长显著受损，表明VdOGDH对营养生长和碳利用至关重要。Delta VdOGDH突变体的分生孢子呈螺旋状圆形或球形，菌丝不规则分枝，缺乏典型的螺旋状分枝。突变体Δ VdOGDH-1和Δ VdOGDH-2对培养基平板中的H2 O2高度敏感，并且具有较高的细胞内ROS水平。Delta VdOGDH突变体也具有氧化反应相关基因的表达升高，表明VdOGDH参与对氧化应激的反应。此外，VdOGDH的破坏导致能量代谢相关基因VdICL、VdICDH、VdMDH和VdPDH以及黑色素相关基因Vayg 1、VdSCD、VdLAC、VT 4 HR和VaflM在Delta VdOGDH突变体中的表达显著增加;因此，VdOGDH对于能量代谢和黑色素积累也很重要。与野生型和Delta VdOGDH-C菌株相比，接种Delta VdOGDH突变体的棉花植株表现出轻度的叶片失绿症，并且病情指数较低。这些结果共同表明VdOGDH参与大丽轮枝菌的能量代谢，也是通过调节多种真菌发育因子来实现完全毒力所必需的。
Verticillium dahliae, a soil-borne fungus, can invade plant vascular tissue and cause Verticillium wilt. The enzyme alpha-oxoglutarate dehydrogenase (OGDH), catalyzing the oxidation of alpha-oxoglutarate in the tricarboxylic acid cycle (TCA), is vital for energy metabolism in the fungi. Here, we identified the OGDH gene in V. dahliae (VdOGDH, VDAG_10018) and investigated its function in virulence by generating gene deletion mutants (Delta VdOGDH) and complementary mutants (Delta VdOGDH-C). When the Delta VdOGDH mutants were supplemented with different carbon sources, vegetative growth on Czapek Dox medium was significantly impaired, suggesting that VdOGDH is crucial for vegetative growth and carbon utilization. Conidia of the Delta VdOGDH mutants were atypically rounded or spherical, and hyphae were irregularly branched and lacked typical whorled branches. Mutants Delta VdOGDH-1 and Delta VdOGDH-2 were highly sensitive to H2O2 in the medium plates and had higher intracellular ROS levels. Delta VdOGDH mutants also had elevated expression of oxidative response-related genes, indicating that VdOGDH is involved in response to oxidative stress. In addition, the disruption of VdOGDH caused a significant increase in the expression of energy metabolism-related genes VdICL, VdICDH, VdMDH, and VdPDH and melanin-related genes Vayg1, VdSCD, VdLAC, VT4HR, and VaflM in the Delta VdOGDH mutants; thus, VdOGDH is also important for energy metabolism and melanin accumulation. Cotton plants inoculated with Delta VdOGDH mutants exhibited mild leaf chlorosis and the disease index was lower compared with wild type and Delta VdOGDH-C strains. These results together show that VdOGDH involved in energy metabolism of V. dahliae, is also essential for full virulence by regulating multiple fungal developmental factors.